Medial Preoptic Area Modulates Courtship Ultrasonic Vocalization in Adult Male Mice
Medial Preoptic Area Modulates Courtship Ultrasonic Vocalization in Adult Male Mice
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内侧视前区调节成年雄性小鼠求偶超声波发声
DOI：
10.1007/s12264-019-00365-w
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发表时间：
2019-08-01
影响因子：
5.6
通讯作者：
Xu, Xiao-Hong
中科院分区：
文献类型：
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作者：
Gao, Shu-Chen;Wei, Yi-Chao;Xu, Xiao-Hong
成年雄性小鼠发出高度复杂的超声波发声（USVs），以响应雌性同种。这种USV被认为有助于求爱行为，通常被作为神经发育和精神疾病（如自闭症）小鼠模型的行为指数进行测量。虽然遗传因素对USV的调控已经得到了广泛的表征，但控制USV产生的神经机制在很大程度上仍然未知。在这里，我们报告说，光遗传激活的内侧视前区（mPOA）引起的生产的USVs，是声学上类似的求爱USVs在成年小鼠。此外，mPOA囊泡GABA转运蛋白阳性（Vgat +）神经元更有效地驱动USV生产比囊泡谷氨酸转运蛋白2阳性神经元。此外，消融mPOA Vgat+神经元导致目标男性USVs的频谱特征和音节使用发生改变。总之，这些结果表明，mPOA在调节求偶USV中起着至关重要的作用，这可能是未来解剖USV产生背后的神经回路的切入点。
Adult male mice emit highly complex ultrasonic vocalizations (USVs) in response to female conspecifics. Such USVs, thought to facilitate courtship behaviors, are routinely measured as a behavioral index in mouse models of neurodevelopmental and psychiatric disorders such as autism. While the regulation of USVs by genetic factors has been extensively characterized, the neural mechanisms that control USV production remain largely unknown. Here, we report that optogenetic activation of the medial preoptic area (mPOA) elicited the production of USVs that were acoustically similar to courtship USVs in adult mice. Moreover, mPOA vesicular GABA transporter-positive (Vgat +) neurons were more effective at driving USV production than vesicular glutamate transporter 2-positive neurons. Furthermore, ablation of mPOA Vgat+ neurons resulted in altered spectral features and syllable usage of USVs in targeted males. Together, these results demonstrate that the mPOA plays a crucial role in modulating courtship USVs and this may serve as an entry point for future dissection of the neural circuitry underlying USV production.