Cell proliferation of estrogen-sensitive cells: the case for negative control.
Cell proliferation of estrogen-sensitive cells: the case for negative control.
复制标题
DOI：
10.1210/edrv-8-1-44
复制
发表时间：
1987-02
影响因子：
20.3
通讯作者：
A. Soto;C. Sonnenschein
中科院分区：
文献类型：
--
作者：
A. Soto;C. Sonnenschein
对切除卵巢的脊椎动物使用雌激素会导致第二性器官中某些类型细胞的增殖，这一观点几十年来一直得到承认(1)。17β-雌二醇（E2）是否直接或间接作用于这些靶细胞的增殖，一直存在广泛的争论，但没有得出确凿的答案。由于这个问题的明确答案不能用整个动物得到，研究人员探索了在培养中外植啮齿动物靶组织的模型（2-4）。然而，这些实验设计没有足够的歧视性，无法得出增殖是否直接归因于雌激素与受刺激细胞的相互作用，还是细胞类型之间或细胞内部的中间步骤导致了所观察到的效果（1-10）。在60年代和70年代，随着雌激素受体（estr）的概念的出现，对整个动物模型中雌激素靶组织增殖反应的表征重新产生了兴趣。
THAT the administration of estrogens to oophorectomized vertebrates results in the proliferation of certain cell types in secondary sex organs has been acknowledged for decades (1). Whether 17β-estradiol (E2) acts directly or indirectly on the proliferation of such target cells has been widely debated without reaching a conclusive answer. Since a definitive answer to this question cannot be obtained using whole animals, researchers explored models in which rodent target tissues have been explanted in culture (2–4). However, these experimental designs were not sufficiently discriminatory to conclude whether proliferation was due directly to the estrogen interaction with the cell that was stimulated or whether intermediate steps among cell types or within cells were responsible for the effect observed (1–10). During the 60's and 70's renewed interest in characterizing the proliferative response of estrogen target tissues in whole animal models became apparent as the concept of the estrogen receptor, or estr...