No changes in neocortical cell volumes or glial cell numbers in chronic alcoholic subjects compared to control subjects
No changes in neocortical cell volumes or glial cell numbers in chronic alcoholic subjects compared to control subjects
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DOI：
10.1093/alcalc/agm007
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发表时间：
2007-09-01
影响因子：
2.8
通讯作者：
Pakkenberg, Bente
中科院分区：
文献类型：
--
作者：
Fabricius, Katrine;Pakkenberg, Henning;Pakkenberg, Bente
目的：研究与对照组受试者相比，有严重酒精滥用史的受试者新皮质中的总胶质细胞群是否完整。进一步研究慢性酒精中毒患者的皮质神经细胞核和神经细胞胞体体积是否与对照组相同。研究方法：采用体视学方法，采用垂直设计的旋光仪，对11名酗酒者和10名正常人的大脑皮层及其四个分区的核周细胞体积和核细胞体积进行了研究。使用Cavalieri估计量和细胞计数的光学disector，我们估计神经胶质细胞在新皮层的总数，并比较以前的体视学结果慢性酒精中毒。结果如下：我们发现，与对照组相比，慢性酒精中毒受试者的平均神经元细胞体积不受严重酒精滥用（p=0.84）和正常神经胶质细胞总数（p=0.39）的影响。结论：到目前为止，只有神经胶质细胞和树突/突触的变化被报道在酒精中毒受试者的大脑的体视学研究。因此，我们有越来越多的证据表明，一些人在停止饮酒后可能会恢复到以前的认知能力，这可能会给慢性酒精成瘾者带来希望和鼓励，以停止滥用。
Aims: To study if the total glial cell population in the neocortex is intact in subjects with a history of severe alcohol abuse compared to control subjects. Further, to investigate whether the cortical nerve cell nuclei and nerve cell perikarya volumes are the same in chronic alcoholic subjects as in the control subjects. Methods: Using the stereological method, the optical rotator in a vertical design, the perikaryon cell volume and nuclei cell volume in the neocortex and its four subdivisions were studied in 11 alcoholics and 10 control subjects. Using the Cavalieri estimator of volumes and the optical disector for cell counting, we estimated the total number of glial cells in the neocortex and compared previous stereological results for chronic alcoholic subjects. Results: We found the mean neuronal cell volumes to be unaffected by severe alcohol abuse (p=0.84) and a normal total number of glial cells (p=0.39) in chronic alcoholic subjects compared to control subjects. Conclusion: Only glial cells and dendritic/synaptic changes have so far been reported in stereological studies of the brains of alcoholic subjects. We thus have increasing evidence that it may be possible for some individuals to return to their previous cognitive abilities after cessation of alcohol which may give hope and encouragement for chronic alcoholic subjects to stop the abuse.