Atrial natriuretic peptide gene expression in the rat gastrointestinal tract.
Atrial natriuretic peptide gene expression in the rat gastrointestinal tract.
复制标题
大鼠胃肠道中心房钠尿肽基因的表达。
DOI：
10.1006/bbrc.1994.1965
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发表时间：
1994
影响因子：
3.1
通讯作者：
Chalfant,CE
中科院分区：
文献类型：
--
作者：
GowerJr,WR;Dietz,JR;Vesely,DL;Finley,CL;Skolnick,KA;Fabri,PJ;Cooper,DR;Chalfant,CE
大鼠胃肠道中 ANP 激素原免疫反应性的存在表明它可能是 ANP 合成的心外部位。本研究的目的是研究成年大鼠胃肠道中 ANP mRNA 的表达。通过核糖核酸酶保护分析检测胃、小肠和大肠以及直肠/肛门中的 ANP mRNA。在近端胃、胃窦、近端结肠和直肠/肛门中发现 ANP 转录物浓度最高，其水平为心室中的 1% 至 10%。对这些组织的总 RNA 进行 Northern 印迹分析，发现了一个 0.9 kb ANP 转录物，与在心脏中检测到的转录物相似。组织提取物的凝胶过滤色谱为近端胃、胃窦、近端结肠和直肠/肛门中存在完整的 ANP 激素原提供了证据。这些结果表明，ANP 基因在大鼠胃肠道的特定区域表达，表明 ANP 合成的组织特异性差异调节发生在胃肠道内。
The presence of ANP prohormone immunoreactivity in rat GI tract suggests that it may be an extracardiac site of ANP synthesis. The aim of this study was to investigate the expression of ANP mRNA in the adult rat GI tract. ANP mRNA was detected by ribonuclease protection analysis in stomach, small and large intestines, and rectum/anus. The highest concentrations of ANP transcripts were found in the proximal stomach, antrum, proximal colon, and rectum/anus at levels that ranged from 1 to 10% of that found in cardiac ventricle. Northern blot analysis of total RNA from these tissues identified a single 0.9 kb ANP transcript similar to that detected in heart. Gel filtration chromatography of tissue extracts provided evidence for the presence of the complete ANP prohormone in proximal stomach, antrum, proximal colon and rectum/anus. These results demonstrate that the gene for ANP is expressed in specific regions of the rat GI tract, suggesting that tissue-specific differential regulation of ANP synthesis occurs within the GI tract.