Multiple Particle Tracking in 3-D+ Microscopy: Method and Application to the Tracking of Endocytosed Quantum Dots
Multiple Particle Tracking in 3-D+ Microscopy: Method and Application to the Tracking of Endocytosed Quantum Dots
复制标题
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Auguste Genovesio;T. Liedl;V. Emiliani;W. Parak;M. Coppey-Moisan;Senior Member Ieee Jean-Christophe Olivo-Marin;J. Olivo-Marin
中科院分区：
文献类型：
--
作者：
Auguste Genovesio;T. Liedl;V. Emiliani;W. Parak;M. Coppey-Moisan;Senior Member Ieee Jean-Christophe Olivo-Marin;J. Olivo-Marin
-我们提出了一种方法来检测和跟踪多个移动的生物点状粒子，这些粒子在通过多维荧光显微镜获得的图像序列中显示出不同种类的动力学。它能够提取和分析诸如内体颗粒的数量、位置、速度、运动和扩散相等信息。该方法由几个阶段组成。在三维（3-D）未消差小波变换执行检测阶段之后，我们计算每个检测到的点在下一帧中其未来状态的几个预测。这要归功于交互式多模型（IMM）算法，该算法包含了与不同生物现实运动类型相对应的几个模型。然后，通过基于每个IMM的似然最大化的数据关联算法构建轨道。最后一个阶段包括更新IMM滤波器，以计算当前图像的最终估计并改进对下一个图像的预测。在合成图像数据上验证了该方法的性能，并将其用于表征含有量子点的内吞囊泡的三维运动。
— We propose a method to detect and track multiple moving biological spot-like particles showing different kinds of dynamics in image sequences acquired through multidimensional flu-orescence microscopy. It enables the extraction and analysis of information such as number, position, speed, movement, and diffusion phases of, e.g., endosomal particles. The method consists of several stages. After a detection stage performed by a three-dimensional (3-D) undecimated wavelet transform, we compute, for each detected spot, several predictions of its future state in the next frame. This is accomplished thanks to an interacting multiple model (IMM) algorithm which includes several models corresponding to different biologically realistic movement types. Tracks are constructed, thereafter, by a data association algorithm based on the maximization of the likelihood of each IMM. The last stage consists of updating the IMM filters in order to compute final estimations for the present image and to improve predictions for the next image. The performances of the method are validated on synthetic image data and used to characterize the 3-D movement of endocytic vesicles containing quantum dots.