New surgical model of post-traumatic osteoarthritis: Isolated intra-articular bone injury in the rabbit
New surgical model of post-traumatic osteoarthritis: Isolated intra-articular bone injury in the rabbit
复制标题
DOI：
10.1002/jor.22284
复制
发表时间：
2013-06-01
影响因子：
2.8
通讯作者：
Frank, Cyril B.
中科院分区：
文献类型：
--
作者：
Huebner, Kyla D.;Shrive, Nigel G.;Frank, Cyril B.
骨关节炎（OA）是世界范围内致残的主要原因。我们假设孤立性关节内骨损伤后的炎症可以刺激创伤后OA，并建立了一个兔子模型来验证这一概念。实验使用了60只雌性新西兰大白兔。26只实验动物在它们的右股骨切口上钻了两个洞，18只兔子做了假手术，16只兔子做了未手术的对照。分别在72、3、6、9、52周对家兔进行亚组安乐死。膝关节进行粗略评估并收集组织。对软骨和滑膜进行组织学和qPCR分析，并进行亚组比较。所有手术关节术后均出现肉眼和组织学（改良Mankin评分）软骨损伤，实验结果随着时间的推移而恶化(p
Osteoarthritis (OA) is a leading cause of disability worldwide. We hypothesized that inflammation following isolated intra-articular bone injury can stimulate post-traumatic OA and developed a rabbit model to test that concept. Sixty female New Zealand White Rabbits were used. Twenty-six experimental animals had two holes drilled into their right femoral-notch, 18 rabbits had sham surgery, and 16 were un-operated controls. Rabbits were euthanized in subgroups at 72h, 3, 6, 9, and 52 weeks. Knees were assessed grossly and tissues collected. Cartilage and synovium were analyzed with histology and qPCR and subgroups compared statistically. All surgical joints showed gross and histological (modified Mankin score) cartilage damage after surgery, with experimentals worsening with time (p