Phycoerythrins in the southern tropical and equatorial Pacific Ocean: Evidence for new cyanobacterial types
Phycoerythrins in the southern tropical and equatorial Pacific Ocean: Evidence for new cyanobacterial types
复制标题
热带南部和赤道太平洋中的藻红蛋白：新蓝藻类型的证据
DOI：
10.1029/98jc02000
复制
发表时间：
1999
影响因子：
--
通讯作者：
J. Blanchot
中科院分区：
文献类型：
--
作者：
J. Neveux;F. Lantoine;D. Vaulot;D. Marie;J. Blanchot
本文对赤道中南部热带和赤道太平洋的S藻红蛋白(PE)进行了定量和定性的研究。我们主要观察到与小聚球藻有关的高植物结合胆素(PUB)PE。(0.6-L.4骨灰盒)。该PE在50%甘油溶液中的荧光激发峰位于496和550 nm。最高浓度(0.1-0.2ug L-L)出现在混合层(富营养区)或0.1um等值线附近(富营养化水体)。聚球藻的最大丰度不超过3 1×10 3细胞·m L-t。流式细胞术未检测到聚球菌亚群。然而，在少数样品中，观察到了两种新的PE光谱类型。第一种类型为高Pub PE，在494和564 nm处有两个荧光激发峰。它似乎归因于不活动的圆形细胞，大小为2-3个骨灰盒，很容易被流式细胞仪检测到，很可能是蓝藻。它们只在海拔60米、沿15“W的15”O以南的极贫营养水域中被观察到。在这一区域，在120米以上没有检测到硝酸盐、亚硝酸盐和氨氮，而磷酸盐总是被记录下来。这表明，这些较大的氰基细菌可能固定氮素(N_2)以供应它们的生长，因此可能在海洋新的生产中发挥重要作用。第二种类型是在赤道附近或附近的三个样品中观察到高藻红素(PEB)PE。它在496和536 nm处有两个荧光激发峰。没有鉴定出含有这种PE类型的生物体。这些数据表明，海洋水域中的PE比之前假设的更加多样化。
Quantitative and qualitative investigations of phycoerythrin s (PE) were achieved in the central southern tropical and equatorial Pacific during the Flux dans l'ouest du Paci- tique equatorial (FLUPAC: September-October 1994) and Oligotrophie en Pacifique (OLIPAC: November 1994) cruises. We observed mainly high-phycourobilin (PUB) PE related to small Synechococcus spp. (0.6-l .4 urn). This PE was characterized by fluorescence excitation peaks at 496 and 550 nm in 50% glycerol. Highest concentrations (0.1-0.2 ug L-l) were recorded either in the mixed layer (nutrient-enriched area) or at depth in the vicinity of the 0.1 uM NO,- isopleth (oligotrophic waters). Maximum abundance of Synechococcus did not exceed 3 1 x 103 cell mL-t . No subpopulations of Synechococcus were evidenced by flow cytometry. Nevertheless, in a few samples, two new PE spectral types were observed. The first type was a high-PUB PE with two fluorescence excitation peaks at 494 and 564 nm. It appears to be attributable to nonmotile round cells, 2-3 urn in size and easily detected by flow cytometry, likely cyanobacteria. They were only observed in very oligotrophic waters south of 15"3O'S along 15O"W in the top 60 m. In this area, nitrate, nitrite, and ammonia were undetectable above 120 m, while phosphate was always recorded. This suggests that these larger cyano- bacteria may fix dinitrogen (N2) to supply their growth and therefore possibly play a signi- ficant role in oceanic new production. The second type, a high-phycoeryt hrobilin (PEB) PE was observed in three samples obtained at or near the equator. It displayed two fluorescence excitation peaks at 496 and 536 nm. The organisms that contained this PE type were not identified. These data suggest that PE is more diverse in oceanic waters than was previously assumed.
藻红蛋白在海洋超微型浮游生物聚球藻中的作用。
DOI：
10.1126/science.3097824
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yeh,SW;Ong,LJ;Glazer,AN
通讯作者：
Glazer,AN