miR-99a and-99b inhibit cervical cancer cell proliferation and invasion by targeting mTOR signaling pathway
miR-99a and-99b inhibit cervical cancer cell proliferation and invasion by targeting mTOR signaling pathway
复制标题
DOI：
10.1007/s12032-014-0934-3
复制
发表时间：
2014-05-01
期刊：
影响因子：
3.4
通讯作者：
Li, Mu
中科院分区：
文献类型：
--
作者：
Wang, Li;Chang, Lihua;Li, Mu
MicroRNA在基因表达调控中发挥着重要作用。在此，我们发现miR-99 a和-99 B（miR-99 a/B）在人宫颈癌患者组织中下调，并且与淋巴转移呈负相关。此外，过表达miR-99 a/B抑制细胞生长和侵袭，而抑制miR-99 a/B产生相反的表型。双荧光素酶报告分析表明mTOR是miR-99 a和-99b的新靶基因。综上所述，miR-99 a/B直接负调控宫颈癌细胞中mTOR的表达，并增强了miR-99 a/B及其靶点在宫颈癌恶性表型中的重要性。
MicroRNAs were demonstrated to play an important role in the regulation of gene expression. Here, we showed that miR-99a and -99b (miR-99a/b) were down-regulated in human cervical cancer patient tissues and were negatively related with lymphatic metastasis. In addition, overexpression of miR-99a/b inhibited cell growth and invasion, whereas suppression of miR-99a/b yielded the reverse phenotype. Dual luciferase report assay revealed that mTOR was identified as a novel target gene of both miR-99a and -99b. Altogether, these results suggested that miR-99a/b directly and negatively regulated mTOR expression in cervical cancer cells, and enforced the importance of miR-99a/b and their targets in the malignant phenotypes of cervical carcinogenesis.