EFFECT OF GONADOTROPHIC HORMONES ON GLUCOSE METABOLISM BY LUTEINIZED RAT OVARIES
EFFECT OF GONADOTROPHIC HORMONES ON GLUCOSE METABOLISM BY LUTEINIZED RAT OVARIES
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DOI：
10.1210/endo-70-5-701
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发表时间：
1962-01-01
期刊：
影响因子：
4.8
通讯作者：
GREEP, RO
中科院分区：
文献类型：
--
作者：
ARMSTRONG, DT;GREEP, RO
纯化的LH已被证明对黄体化大鼠卵巢切片的体外葡萄糖摄取速率具有明显的刺激作用。这种效应在静脉注射激素后30分钟内变得明显，但不能通过将激素直接添加到孵育培养基中来产生。整个卵巢和四分卵巢利用葡萄糖的速度比薄切片，但仍然响应LH刺激体内。其他促性腺激素，FSH和催乳素，没有产生刺激作用，这不能解释LH污染的基础上。LH不能刺激肾上腺、肾脏、膈肌、胸腺、子宫和脂肪组织的葡萄糖摄取。LH对卵巢葡萄糖摄取的刺激伴随着葡萄糖-1-C14和葡萄糖-6-C14转化为C14 O2和Cl 4-脂质的不太明显的增加。在未受刺激和LH刺激的黄体化卵巢中，CO2和脂质中葡萄糖的碳1以与碳6基本相同的速率出现，表明非常少的葡萄糖被磷酸己糖旁路代谢。LH增加葡萄糖摄取速率的能力与其降低卵巢抗坏血酸浓度的能力密切相关，表明这两种卵巢对LH的反应之间存在功能相关性。
Purified LH has been shown to exert a pronounced stimulatory action upon the rate of in vitro glucose uptake by slices of luteinized rat ovaries. This effect became evident within 30 minutes after intravenous injection of the hormone, but could not be produced by addition of the hormone directly to the incubation media. Whole ovaries and quartered ovaries utilized glucose less rapidly than did thin slices, but were still responsive to LH stimulation in vivo. The other gonadotrophins, FSH and prolactin, produced no stimulatory effects that could not be explained on the basis of LH contamination. LH failed to stimulate glucose uptake by adrenal, kidney, diaphragm, thy-mus, uterus and adipose tissue. The stimulation of ovarian glucose uptake by LH was accompanied by a less pronounced increase in con -version of glucose- 1-C14 and glucose-6-C14 to C14O2 and to Cl4-lipids. Carbon 1 of glucose appeared in both CO2 and lipids at essentially the same rate as carbon 6, both in unstimulated and in LH-stimulated luteinized ovary, suggesting that very little glucose was being metabolized by the hexose monophosphate shunt pathway. The ability of LH to increase the rate of glucose uptake closely paralleled its ability to reduce the ovarian ascorbic acid concentration, suggesting a functional correlation between these two ovarian responses to LH.