A novel in vivo method to detect DNA sequence variation.
A novel in vivo method to detect DNA sequence variation.
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一种检测 DNA 序列变异的新型体内方法。
DOI：
10.1101/gr.5.5.474
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发表时间：
1995
期刊：
影响因子：
7
通讯作者：
Cox,DR
中科院分区：
文献类型：
--
作者：
Faham,M;Cox,DR
错配修复检测 (MRD) 是一种体内方法，利用细菌菌落颜色的变化来检测 DNA 序列变异。将待筛选变异的 DNA 片段克隆到两个 MRD 质粒中，并用这些构建体的异源双链体转化细菌。所得菌落在不存在错配的情况下呈蓝色，在存在错配的情况下呈白色。 MRD 能够检测大小达 10 kb 的 DNA 片段中的单个错配。此外，MRD 具有同时分析多个片段的潜力，为大基因组区域的高通量基因分型和突变检测提供了强大的方法。
Mismatch repair detection (MRD) is an in vivo method that uses a change in bacterial colony color to detect DNA sequence variation. DNA fragments to be screened for variation are cloned into two MRD plasmids, and bacteria are transformed with heteroduplexes of these constructs. The resulting colonies are blue in the absence of a mismatch and white in the presence of a mismatch. MRD is capable of detecting a single mismatch in a DNA fragment as large as 10 kb in size. In addition, MRD has the potential for analyzing many fragments simultaneously, offering a powerful method for high-throughput genotyping and mutation detection in a large genomic region.