Genome scan for cis-regulatory DNA motifs associated with social behavior in honey bees
Genome scan for cis-regulatory DNA motifs associated with social behavior in honey bees
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DOI：
10.1073/pnas.0607448103
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发表时间：
2006-10-31
影响因子：
11.1
通讯作者：
Robinson, Gene E.
中科院分区：
文献类型：
--
作者：
Sinha, Saurabh;Ling, Xu;Robinson, Gene E.
蜜蜂（Apis mellifera）经历了一个与年龄相关的、受社会调节的从在蜂巢中工作到觅食的转变，这与大脑中数千个基因表达的变化有关。为了开始研究基因表达的大规模社会调节背后的顺式调控密码，我们使用新测序的蜜蜂基因组扫描了8组行为相关基因的启动子区域，这些基因在工蜂分工的背景下在大脑中差异表达，其中41个顺式调控基序先前在果蝇中表征。已知在果蝇神经系统发育、嗅觉学习或激素结合中起作用的转录因子Hairy、GAGA、Adf 1、Cf 1、Snail和Dri的结合位点与一个或多个基因集显著相关。一些结合位点的存在也预测了一些基因集中多达71%的基因的表达模式。这些结果表明，大脑基因表达的顺式调节和社会调节之间存在强大的关系，特别是考虑到我们研究了所有已知转录因子的< 15%。这些结果还表明，参与果蝇发育调控的转录网络被用来调控成年蜜蜂的行为发育。然而，这两个过程之间的基因调控的差异建议的发现，行为相关的蜜蜂基因的启动子区域不同的基序发生和G/C含量相对于他们的果蝇直系同源物。
Honey bees (Apis mellifera) undergo an age-related, socially regulated transition from working in the hive to foraging, which is associated with changes in the expression of thousands of genes in the brain. To begin to study the cis-regulatory code underlying this massive social regulation of gene expression, we used the newly sequenced honey bee genome to scan the promoter regions of eight sets of behaviorally related genes differentially expressed in the brain in the context of division of labor among worker bees, for 41 cis-regulatory motifs previously characterized in Drosophila melanogaster. Binding sites for the transcription factors Hairy, GAGA, Adf1, Cf1, Snail, and Dri, known to function in nervous system development, olfactory learning, or hormone binding in Drosophila, were significantly associated with one or more gene sets. The presence of some binding sites also predicted expression patterns for as many as 71% of the genes in some gene sets. These results suggest that there is a robust relationship between cis and social regulation of brain gene expression, especially considering that we studied < 15% of all known transcription factors. These results also suggest that transcriptional networks involved in the regulation of development in Drosophila are used to regulate behavioral development in adult honey bees. However, differences in gene regulation between these two processes are suggested by the finding that the promoter regions for the behaviorally related bee genes differed in both motif occurrence and G/C content relative to their Drosophila orthologs.