Micellar Extraction and Preconcentration of Anthraquinone Derivatives from Rhubarb Prior to Their HPLC‐DAD Determination
Micellar Extraction and Preconcentration of Anthraquinone Derivatives from Rhubarb Prior to Their HPLC‐DAD Determination
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DOI：
10.1080/10826070601064474
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发表时间：
2007-01
影响因子：
1.3
通讯作者：
Zhihong Shi;Xiaomin Zhu;Q. Cheng;Hongyi Zhang
中科院分区：
文献类型：
--
作者：
Zhihong Shi;Xiaomin Zhu;Q. Cheng;Hongyi Zhang
摘要 本文在 HPLC 分析之前，采用非离子表面活性剂低聚乙二醇单烷基醚 (Genapol X-080) 对大黄中的五种蒽醌衍生物进行胶束萃取和预浓缩。研究了各种实验条件以优化提取工艺。在最佳条件下，即10% Genapol X-080（w/v）、液固比50:1（mL·g−1）、超声辅助提取45 min时，蒽醌衍生物的提取率达到最高值。采用浊点萃取法（CPE）预浓缩蒽醌衍生物时，将溶液在55℃恒温水浴中孵育45 min，并在溶液中加入0.24 g·mL−1硫酸钠，以促进CPE过程中的相分离并增加预浓缩系数。与常用的有机溶剂相比，10% Genapol X-080 的蒽醌衍生物提取率最高。本文建立的胶束萃取和预浓缩方法被证明是传统有机溶剂萃取方法的一种简单有效的环境友好替代方法。
Abstract In this paper, the nonionic surfactant, oligoethylene glycol monoalkyl ether (Genapol X‐080), was employed for the micellar extraction and preconcentration of the five anthraquinone derivatives from rhubarb prior to HPLC analysis. Various experimental conditions were investigated to optimize the extraction process. Under optimum conditions, i.e., 10% Genapol X‐080 (w/v), liquid/solid ratio of 50:1 (mL · g−1), ultrasonic assisted extraction for 45 min, the extraction yield of anthraquinone derivatives reached the highest value. For the preconcentration of anthraquinone derivatives by cloud point extraction (CPE), the solution was incubated in a thermostatic water bath at 55°C for 45 min, and 0.24 g · mL−1 sodium sulfate was added to the solution to facilitate the phase separation and increase the preconcentration factor during the CPE process. Compared with commonly used organic solvents, 10% Genapol X‐080 has the highest extraction yield of anthraquinone derivatives. The micellar extraction and preconcentration method established in this paper is demonstrated to be a simple and effective environment friendly alternative to conventional organic solvent extraction methods.