Identification of prolactin receptors in hepatic nuclei.
Identification of prolactin receptors in hepatic nuclei.
复制标题
肝细胞核中催乳素受体的鉴定。
DOI：
10.1016/0003-9861(92)90563-c
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发表时间：
1992
影响因子：
3.9
通讯作者：
Putnam,CW
中科院分区：
文献类型：
--
作者：
Buckley,AR;Montgomery,DW;Hendrix,MJ;Zukoski,CF;Putnam,CW
催乳素是一种营养激素，可直接作用于肝细胞核。在这项研究中，在纯化的大鼠肝核中寻找特定的催乳素结合位点。饱和且特异的高亲和力 125 I-催乳素结合位点被证明位于细胞核上或细胞核内。催乳素结合被大鼠和绵羊催乳素竞争性抑制，但不被大鼠生长激素抑制。使用免疫金电子显微镜，我们检测到整个细胞核中与异染色质相关的催乳素受体。此外，内源性免疫反应性催乳素被证明存在于肝细胞核内。我们得出的结论是，大鼠肝细胞核具有催乳素结合位点，可能参与激素引导的生长过程。
Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.