Hypothalamic levels of NPY, MCH, and prepro-orexin mRNA during pregnancy and lactation in the rat:: role of prolactin
Hypothalamic levels of NPY, MCH, and prepro-orexin mRNA during pregnancy and lactation in the rat:: role of prolactin
复制标题
DOI：
10.1096/fj.02-0933com
复制
发表时间：
2003-08-01
期刊：
影响因子：
4.8
通讯作者：
Señarís, RM
中科院分区：
文献类型：
--
作者：
García, MC;López, M;Señarís, RM
怀孕和哺乳期提供了生理性食欲亢进和高催乳素血症的良好模型。为了确定在这些情况下与增加进食相关的可能因素，我们通过原位杂交测量了非妊娠、妊娠和哺乳期大鼠中三种促食欲神经肽（NPY、MCH 和食欲素）的下丘脑 mRNA 水平。妊娠期和哺乳期弓状核中 NPY mRNA 含量显着增加。然而，在这两种状态下，MCH 和前食欲素原表达均下降。妊娠和哺乳期大鼠禁食48或72小时进一步升高NPY mRNA水平并增加低MCH mRNA含量。令人惊讶的是，未观察到前食欲素原 mRNA 水平受到影响。最后，我们使用垂体移植物诱导的高催乳素血症模型研究了高 PRL 水平对这些促食欲信号的可能影响。 NPY mRNA 含量没有变化，但 MCH 和前食欲素原 mRNA 水平显着降低。我们的结果表明，NPY 表达增加可能是妊娠和哺乳期间观察到的食欲亢进的部分原因。 MCH 和前食欲素原可能参与其他稳态机制的适应，并且它们在这些生理环境中水平的降低可能是由循环 PRL 水平升高介导的。
Pregnancy and lactation provide excellent models of physiological hyperphagia and hyper-prolactinemia. To identify possible factors associated with the increased feeding in these situations, we measured hypothalamic mRNA levels of three orexigenic neuropeptides - NPY, MCH, and orexins - in nonpregnant, pregnant, and lactating rats by in situ hybridization. NPY mRNA content in the arcuate nucleus was significantly increased during pregnancy and lactation. However, MCH and prepro-orexin expression was decreased in both states. 48 or 72 h of fasting in pregnant and lactating rats further elevated NPY mRNA levels and increased the low MCH mRNA content. Surprisingly, no effect was observed in preproorexin mRNA levels. Finally, we investigated the possible effect of high PRL levels on these orexigenic signals using a model of hyperprolactinemia induced by pituitary graft. NPY mRNA content was unchanged, but MCH and prepro-orexin mRNA levels were significantly decreased. Our results suggest that the increased NPY expression might be partly responsible for the hyperphagia observed during pregnancy and lactation. MCH and prepro-orexin may be involved in the adaptation of other homeostatic mechanisms and their decreased levels in these physiological settings could be mediated by the elevated circulating PRL levels.