TRIPLET ABSORPTION AND PHOSPHORESCENCE EMISSION IN ZINC CYTOCHROME-C
TRIPLET ABSORPTION AND PHOSPHORESCENCE EMISSION IN ZINC CYTOCHROME-C
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DOI：
10.1016/0014-5793(81)81002-2
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
VANDERKOOI, JM
中科院分区：
文献类型：
--
作者：
DIXIT, SN;WARING, AJ;VANDERKOOI, JM
由于卟啉的激发态性质与理解生物体系中的电子转移过程密切相关，因此受到了广泛的关注。激发态卟啉可以在模型体系如Ni（II）、Cu（II）、Mg（II）、Ag（II）、Zn（II）、Pd（II）和Pt（II）的卟啉衍生物中转移电子[1 - 5]。在关于铜细胞色素c的稳态和时间分辨发光发射的报告[6]中，低于80 K的分子内速率参数受到卟啉-蛋白质相互作用的显著影响。取代卟啉的另一个应用是研究膜系统中的整个分子旋转运动。[6]的文件。研究了铑取代的细胞色素b5的三重态，并将其性质用于测量分子在磷脂囊泡系统中的旋转扩散[71]。在这里，我们报告的结果，我们的测量瞬态三重态吸收和磷光发射的衰减时间在锌细胞色素c。已知锌卟啉可以在模型系统中的激发态转移电子[8]。我们的目的是调查是否类似的电子转移发生在蛋白质含有锌取代卟啉。
The excited state properties of porphyrins have attracted considerable attention due to their relevance to the understanding of electron transfer processes in biological systems. The excited state porphyrins can transfer electrons in model systems such as the porphyrin derivatives of Ni (II), Cu (II), Mg (II), Ag (II), Zn (II), Pd (I1) and Pt (I1)[l-5]. In the report [6] on the steady-state and time-resolved luminescence emission in copper cytochrome c, intramolecular rate parameters below 80 K were significantly influenced by the porphyrin-protein interaction. Another application of substituted porphyrins is the study of the whole molecule rotational motion in membrane systems.[6]. The triplet state of rhodium-substituted cytochrome b5 was examined and its properties used to measure the rotational diffusion of the molecule in phospholipid vesicle system [71. Here we report the results of our measurements of decay time of transient triplet absorption and phosphorescence emission in zinc cytochrome c. It is known that zinc porphyrins can transfer electrons in the excited state in model systems [8]. We aim to investigate whether similar electron transfer occurs in proteins containing zinc-substituted porphyrins.