E-cadherin mediates adhesion and suppresses cell motility via distinct mechanisms.
E-cadherin mediates adhesion and suppresses cell motility via distinct mechanisms.
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DOI：
10.1242/jcs.110.3.345
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发表时间：
1997-02
影响因子：
4
通讯作者：
Haiyan Chen;N. Paradies;M. Fedor-Chaiken;R. Brackenbury
中科院分区：
文献类型：
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作者：
Haiyan Chen;N. Paradies;M. Fedor-Chaiken;R. Brackenbury
钙依赖性粘附分子E-cadherin的表达抑制体外细胞的侵袭，但这种作用的机制尚不清楚。为了研究这一机制，我们分析了在小鼠L细胞和大鼠星形胶质细胞样WC 5细胞中表达E-cadherin的影响。E-钙粘蛋白介导的细胞粘附增加减少了在WC 5细胞和一些L-细胞中的侵袭，但在其他细胞中则不然。在所有的情况下，抑制侵袭与减少细胞运动在体外伤口填充试验和transwell运动试验评估。为了确定E-钙粘蛋白介导的粘附和运动抑制之间的关系，我们分析了删除E-钙粘蛋白胞质结构域的不同区域的影响。缺乏整个胞质结构域的E-钙粘蛋白不介导钙依赖性粘附，并没有减少细胞运动时，在WC 5细胞中表达。缺乏一部分连环蛋白结合域的E-钙粘蛋白不与细胞骨架，并没有促进粘附，但仍然抑制运动的WC 5细胞。此外，E-钙粘蛋白，保留了完整的连环蛋白结合域，但缺乏胞质结构域的质膜部分，介导有效的粘附，但不抑制运动。这些结果表明，E-cadherin介导的粘附和抑制细胞的运动，通过不同的E-cadherin在抑制运动中发挥关键作用。
Expression of the calcium-dependent adhesion molecule E-cadherin suppresses the invasion of cells in vitro, but the mechanism of this effect is unknown. To investigate this mechanism, we analyzed the effects of expressing E-cadherin in mouse L-cells and rat astrocyte-like WC5 cells. Increased cellular adhesion mediated by E-cadherin reduced invasion in WC5 cells and in some L-cells, but not in others. In all cases, suppression of invasion was correlated with decreased cell movement as assessed in an in vitro wound-filling assay and a transwell motility assay. To define the relationship between adhesion mediated by E-cadherin and suppression of motility, we analyzed the effects of deleting different regions of the E-cadherin cytoplasmic domain. E-cadherin lacking the entire cytoplasmic domain did not mediate calcium-dependent adhesion and did not reduce cell motility when expressed in WC5 cells. E-cadherin lacking a portion of the catenin-binding domain did not associate with the cytoskeleton and did not promote adhesion, yet still suppressed the motility of WC5 cells. In addition, E-cadherin that retains an intact catenin-binding domain, but lacks a juxtamembrane portion of the cytoplasmic domain, mediated effective adhesion, but did not suppress motility. These results indicate E-cadherin mediates adhesion and suppresses cell motility via distinct of E-cadherin plays a key role in suppressing motility.