Nuclear protein content and Ki-67 immunoreactivity in nonneoplastic and neoplastic thyroid cells.
Nuclear protein content and Ki-67 immunoreactivity in nonneoplastic and neoplastic thyroid cells.
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非肿瘤性和肿瘤性甲状腺细胞中的核蛋白含量和 Ki-67 免疫反应性。
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发表时间：
1992
期刊：
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通讯作者：
G. Auer
中科院分区：
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作者：
G. Wallin;M. Bäckdahl;B. Christensson;L. Grimelius;G. Auer
甲状腺肿瘤细胞核DNA含量与肿瘤的恶性潜能密切相关。除了DNA，核蛋白（NP）构成了细胞核的主要物质。与生长停滞的细胞相比，NP含量可能在生长中的增殖阶段显著变化。与从G 0到G1的过渡相关的NP含量的增加发生在DNA合成开始之前，并且可以用于评估生长活性。本文分析了90例非肿瘤性病变、75例良性和62例恶性甲状腺肿瘤细胞核DNA和NP含量。所有非肿瘤标本均为整倍体，90例中有1例为生长激活。良性肿瘤组整倍体59例，异倍体16例。其中，分别有5/59份（9%）和6/16份（38%）生长激活标本。在恶性肿瘤组中，62例中有57例被归类为整倍体，根据NP含量，该组中有12例（21%）显示出生长活性。62例中有5例为非整倍体，其中3例（60%）为生长激活型。通过单克隆抗体Ki-67的生长活性的评价进行了一个亚组的32个甲状腺标本，非肿瘤性和肿瘤性病变。Ki-67免疫反应阳性细胞在6例非肿瘤性病变中为0 ~ 1.1%，在14例良性病变中为0 ~ 3.1%，在12例恶性肿瘤中为0.2-3.9%。NP/DNA比值和Ki-67免疫反应性反映的生长活性在非肿瘤性甲状腺病变和甲状腺肿瘤中均较低。
The nuclear DNA content in thyroid tumor cells has been shown to be closely related to the malignant potential of the neoplasm. Besides DNA, nuclear protein (NP) constitutes the major mass of the nucleus. The NP content may vary significantly in relation to the proliferative stage in growing as compared to growth-arrested cells. The increase in NP content associated with the transition from G0 to G1 occurs before the onset of DNA synthesis and may be used to assess growth activity. The nuclear DNA and NP contents were analyzed in 90 nonneoplastic lesions and 75 benign and 62 malignant thyroid tumors. All nonneoplastic specimens were euploid, and 1 of 90 was growth activated. In the group of benign tumors, 59 were euploid, and 16 were aneuploid. Among these there were 5 (9%) of 59 and 6 (38%) of 16 growth-activated specimens, respectively. In the group of malignant tumors 57 of 62 were classified as euploid, and in this group 12 (21%) showed growth activity according to the NP content. Five of 62 were aneuploid, and 3 (60%) of these 5 tumors were growth activated. Evaluation of the growth activity by means of monoclonal antibody Ki-67 was performed on a subgroup of 32 thyroid specimens, both nonneoplastic and neoplastic lesions. Ki-67 immunoreactivity was observed in 0-1.1% cells of 6 nonneoplastic lesions, in 0-3.1% cells of 14 benign cells and in 0.2-3.9% cells of 12 malignant thyroid tumors. Growth activity, as reflected by the NP/DNA ratio and Ki-67 immunoreactivity, appears to be low both in nonneoplastic thyroid lesions and thyroid tumors.