Identification of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater
Identification of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater
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DOI：
10.1016/j.ibiod.2012.08.003
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发表时间：
2012-11-01
影响因子：
4.8
通讯作者：
Watanabe, Yoshimasa
中科院分区：
文献类型：
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作者：
Miyoshi, Taro;Aizawa, Tomoyasu;Watanabe, Yoshimasa
对膜生物反应器（mbr）处理实际城市污水连续运行过程中引起膜污染的蛋白质进行了详细研究。我们在不同的固体保留时间（srt）下连续运行了两个相同的中试mbr，并在操作结束时提取了杂质。无论操作条件如何，从污染膜中提取的污物中蛋白质是主要成分。对提取的蛋白质进行二维聚丙烯酰胺凝胶电泳（2D-PAGE）分离，并通过n端氨基酸测序分析进行鉴定。超滤（UF）膜粗浓缩和三氯乙酸（TCA）沉淀联合浓缩的蛋白质在2D-PAGE凝胶上分离和可视化效果良好。2D-PAGE分析结果表明，导致膜污染的蛋白质组成因SRT的不同而有显著差异，尽管在氨基酸组成分析中看不到这种差异。通过对选定的2D-PAGE位点进行n端氨基酸测序分析，鉴定出两个特征良好的假单胞菌属外膜蛋白OprF和OprD。据我们所知，这是第一次成功鉴定出在mbr处理实际废水的连续操作中导致膜污染的蛋白质。(C) 2012 Elsevier Ltd.版权所有。
The proteins that caused membrane fouling in a continuous operation of membrane bioreactors (MBRs) treating real municipal wastewater were investigated in detail. We continuously operated two identical pilot-scale MBRs under different solid retention times (SRTs) and extracted the foulants at the end of the operation. Regardless of the operating conditions, proteins were dominant components in the foulants extracted from the fouled membranes. The extracted proteins were subjected to the separation with two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and the identification through the N-terminal amino acid sequencing analysis. The proteins concentrated by the combination of the crude concentration using an ultra-filtration (UF) membrane and trichloroacetic acid (TCA) precipitation were separated and visualized well on 2D-PAGE gels. The results of 2D-PAGE analysis indicated that the compositions of proteins that caused membrane fouling significantly differed depending on the SRT, although such differences cannot be seen in the amino acid composition analysis. Analyzing selected 2D-PAGE spots by N-terminal amino acid sequencing analysis led to the identification of two well-characterized outer membrane proteins originating from Pseudomonas genus, namely OprF and OprD. To our knowledge, this is the first successful identification of proteins that have caused membrane fouling in continuous operations of MBRs treating real wastewater. (C) 2012 Elsevier Ltd. All rights reserved.