Three distinct cell populations in rat kidney collecting duct.
Three distinct cell populations in rat kidney collecting duct.
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大鼠肾集合管中的三种不同的细胞群。
DOI：
10.1152/ajpcell.1987.253.2.c323
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Spicer,SS
中科院分区：
文献类型：
--
作者：
Holthöfer,H;Schulte,BA;Pasternack,G;Siegel,GJ;Spicer,SS
用Na ~+-K ~+-ATP酶和阴离子通道（带3）糖蛋白的免疫细胞化学方法研究了大鼠肾集合管中形态异质的细胞群。这两种酶都定位于收集管各段中单独和形态上不同的细胞亚群的基底面。Na+-K+-ATP酶似乎只存在于主细胞中，通过其形态学鉴定，而带3抗体仅与嵌入细胞反应。然而，5-20%的细胞与闰细胞的形态特征未能与任一抗血清在集合管的各段。由于带3糖蛋白用于将细胞内碳酸氢根交换为氯离子，因此极有可能对该抗原呈阳性的细胞分泌质子。因此，这里介绍的方法似乎是有用的区分主细胞和插入细胞的酶含量的差异，并进一步揭示两个亚群的插入细胞。该方法有望为研究不同代谢状态下的主细胞群和嵌入细胞群提供一种有用的方法。
The morphologically heterogeneous cell populations in the collecting ducts of the rat kidney were studied using immunocytochemical detection of Na+-K+-ATPase and the anion channel (band 3) glycoprotein. Both enzymes were localized to the basal aspect of separate and morphologically distinct subpopulations of cells in various segments of the collecting duct. Na+-K+-ATPase appeared to be present exclusively in principal cells as identified by their morphology, whereas band 3 antibodies reacted only with intercalated cells. However, 5-20% of cells with the morphological characteristics of intercalated cells failed to react with either antisera in various segments of collecting ducts. As band 3 glycoprotein serves in exchanging intracellular bicarbonate for chloride, it is highly likely that the cells positive for this antigen secrete protons. The method introduced here appears thus useful for distinguishing between principal and intercalated cells by differences in their enzyme content and further for revealing two subpopulations of intercalated cells. This method promises to provide a useful approach for studying the principal and intercalated cell populations in various metabolic states.
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兔髓质集合管酸化的阴离子依赖性。
DOI：
10.1172/jci110905
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Stone,DK;Seldin,DW;Kokko,JP;Jacobson,HR
通讯作者：
Jacobson,HR
肾小管中的钠离子和钾离子泵。
影响因子：
33.6
作者：
P. L. Jørgensen
通讯作者：
P. L. Jørgensen
带 3 蛋白在正常和囊性纤维化皮肤中的免疫定位。
DOI：
10.1177/34.6.3517151
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Hazen-Martin,DJ;Pasternack,G;Spicer,SS;Sens,DA
通讯作者：
Sens,DA
从大脑中纯化富含 (Na K )-ATP 酶的内在膜蛋白组分。
DOI：
--
发表时间：
1978
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
K. Sweadner
通讯作者：
K. Sweadner
DOI：
10.1016/s0021-9258(17)43089-4
发表时间：
1984-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Leto;V. Marchesi
通讯作者：
T. Leto;V. Marchesi