A tomato dynein light chain gene SlLC6D is a negative regulator of chilling stress
A tomato dynein light chain gene SlLC6D is a negative regulator of chilling stress
复制标题
番茄动力蛋白轻链基因 SlLC6D 是冷胁迫的负调节因子。
DOI：
10.1016/j.plantsci.2020.110753
复制
发表时间：
2021-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhan, Xiangqiang
中科院分区：
文献类型：
--
作者：
Hu, Tixu;Wang, Shufeng;Zhan, Xiangqiang
动力蛋白轻链（Dynein light chain， DLC）蛋白是动力蛋白复合物的重要组成部分，广泛分布于动植物体内，参与多种细胞过程。DLC基因在植物低温胁迫中的作用尚不清楚。本研究从番茄中分离到一个DLC基因，命名为SlLC6D。启动子分析揭示了SlLC6D启动子中许多参与非生物胁迫的顺式元件。SlLC6D的表达受热、盐胁迫诱导，受聚乙二醇和冷胁迫抑制。在低温胁迫下，番茄SlLC6D基因的下调表现出较低的相对电解质泄漏、丙二醛含量和活性氧积累。低温胁迫下，低表达系脯氨酸含量、超氧化物歧化酶和过氧化物酶活性均高于野生型和过表达系。在低敲系中检测到三个冷响应基因的高转录丰度。低温胁迫下，敲低系的幼苗生长显著高于野生型和过表达系。这些结果表明，SlLC6D可能通过调控ROS含量和ICE1-CBF-COR通路，对低温胁迫耐受性起到负调控作用。
Dynein light chain (DLC) proteins are an important component of dynein complexes, which are widely distributed in plants and animals and involved in a variety of cellular processes. The functions of DLC genes in plant chilling stress remain unclear. In this study, we isolated a DLC gene from tomato, designated SlLC6D. Promoter analysis revealed many cis-elements involved in abiotic stress in the SlLC6D promoter. Expression of SlLC6D was induced by heat and salt stress, and inhibited by polyethylene glycol and chilling stress. Knockdown of SlLC6D in tomato exhibited low relative electrolyte leakage, malondialdehyde content, and reactive oxygen species (ROS) accumulation under chilling stress. The content of proline and activities of superoxide dismutase and peroxidase in knockdown lines were higher than in the wild type and overexpression lines during chilling stress. The high transcript abundances of three cold-responsive genes were detected in knockdown lines in response to chilling stress. Seedling growth of knockdown lines was significantly higher than that of the wild type and overexpression lines under chilling stress. These results suggest that SlLC6D is a negative regulator of chilling stress tolerance, possibly by regulating ROS contents and the ICE1-CBF-COR pathway.