Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells.
Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells.
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DOI：
10.1039/d3cc00364g
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发表时间：
2023-03
影响因子：
4.9
通讯作者：
C. Wong;Lu-Lu Sun-Lu;Meng-Jiao Liu;E. Stride;J. Raymond;Hai-Hao Han;J. Kwan;A. Sedgwick
中科院分区：
文献类型：
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作者：
C. Wong;Lu-Lu Sun-Lu;Meng-Jiao Liu;E. Stride;J. Raymond;Hai-Hao Han;J. Kwan;A. Sedgwick
我们报告的羟基自由基（stecOH）响应荧光探针，利用3，5-二羟基苄基（DHB）功能的合成。4-甲基伞形酮-DHB（Umb-DHB）和试卤灵-DHB（Res-DHB）在stecOH自由基存在下导致它们各自在460 nm和585 nm处的荧光发射强度显著增加。Res-DHB在HeLa细胞中的孵育，随后是治疗性超声（1 MHz），导致荧光发射强度显著增加，从而允许监测活细胞中超声诱导的stecOH产生的能力。
We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells.