The administration of dexmedetomidine changes microRNA expression profiling of rat hearts
The administration of dexmedetomidine changes microRNA expression profiling of rat hearts
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右美托咪定改变大鼠心脏的 microRNA 表达谱
DOI：
10.1016/j.biopha.2019.109463
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发表时间：
2019
影响因子：
7.5
通讯作者：
Qian Jinqiao
中科院分区：
文献类型：
--
作者：
Wang Lingyan;Tang Shumiao;Wang Zhuoran;Chen Hongmei;Rajcha Shiva Sunder;Qian Jinqiao
研究背景右旋美托咪定广泛应用于围手术期和ICU患者。MicroRNAs(MiRNAs)是基因表达的调节因子。方法将6只大鼠随机分为2组(n = 3)：右旋美托咪胺组和对照组。右旋美托咪定组大鼠按100 μg/kg剂量给予右旋美托咪定，对照组大鼠给予生理盐水。麻醉下给予右美托咪定或生理盐水30 min后摘除心脏。用Exiqon miRNA阵列分析差异表达的microRNAs。利用qRT-PCR对差异表达的microRNAs进行了验证。通过基因本体论(GO)和京都基因与基因组百科全书(KEGG)途径分析，寻找异常表达miRNAs的靶基因和信号通路。结果与对照组相比，6个microRNAs显著表达，其中5个microRNAs(miRNA-434-3p、miRNA-3596d、miRNA-496-5p、miRNA-7a-2-3p和miRNA-702-3p)表达上调，1个microRNA(miRNA-208B-3p)表达下调。用实时定量聚合酶链式反应(qRT-PCR)进一步验证了异常表达的microRNAs。GO和KEGG分析用于确定靶基因和信号通路。结论右美托咪定的使用与差异表达的microRNA有关，这可能参与右美托咪定的心脏保护作用。
BackgroundDexmedetomidine is widely used for perioperative and ICU patients. microRNAs (miRNAs) function as regulators of gene expression. The aim of the study was to assay expression profiling of microRNA in rat hearts following administration of dexmedetomidine.MethodsIn this study 6 rats were randomly divided into two groups (n = 3): dexmedetomidine group and control group. The rats of dexmedetomidine group were intraperitoneally given dexmedetomidine in a dose of 100 μg/kg whereas the rats in control group were administered normal saline intraperitoneally. The hearts were excised 30 min after the administration of dexmedetomidine or normal saline under anesthesia. The samples were analyzed for differentially expressed microRNAs with Exiqon miRNA Array. The differentially expressed microRNAs were confirmed by using qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the target genes and signaling pathways of the aberrantly expressed miRNAs.ResultsSix microRNAs were identified to be significantly expressed, among of which, five microRNAs (miRNA-434-3p, miRNA-3596d, miRNA-496-5p, miRNA-7a-2-3p and miRNA-702-3p) were up-regulated and 1 microRNA (miRNA-208b-3p) down-regulated compared to those of control group. The aberrantly expressed microRNAs were further validated by Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). GO and KEGG analyses were used to identify target genes and the signaling pathways.ConclusionsThe use of dexmedetomidine is associated with differentially expressed microRNAs which may be involved in cardioprotection following administration of dexmedetomidine.