STUDY OF DNA SPONTANEOUS DEGRADATION
STUDY OF DNA SPONTANEOUS DEGRADATION
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DOI：
10.1016/0005-2787(76)90174-x
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
VERLY, WG
中科院分区：
文献类型：
--
作者：
CRINE, P;VERLY, WG
DNA分子中完整的无嘌呤中心半衰期为37度。在与细胞内离子组成相近的介质中，C和pH为7.21，在288-335h之间，~3H标记的T7噬菌体DNA在37度时自发降解。在相同的介质中，用氢氧化钠变性后对蔗糖梯度进行沉淀分析，相当于每条DNA链断裂0.009次/h。在孵育的T7噬菌体DNA中发现的完整的无嘌呤结合位点的数量远远低于根据脱嘌呤作用是DNA降解的唯一原因的假设计算的理论值。在实验条件下，排尿显然是DNA降解的一个次要原因。
The intact apurinic site half-life in DNA molecules, at 37.degree. C and pH 7.21, in a medium approximating the intracellular ionic composition, was between 288-335 h. The spontaneous degradation of 3H-labeled T7 phage DNA, at 37.degree. C and pH 7.21, in the same medium, was followed by sedimentation analysis on sucrose gradients after denaturation with NaOH; it was equivalent to 0.009 break/h per DNA strand. The number of intact apurinic sites found in this incubated T7 phage DNA was much lower than the theoretical value calculated on the assumption that depurination was the only cause of DNA degradation. Depurination was apparently a minor cause of DNA degradation in the experimental conditions.