Real-time PCR
Real-time PCR
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DOI：
10.1157/13059826
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发表时间：
2004-05-01
影响因子：
2.5
通讯作者：
Costa, J
中科院分区：
文献类型：
--
作者：
Costa, J
基于PCR的测定目前在大多数微生物学实验室中常规使用。但是，除了少数例外，它们仅限于病毒学领域，特别是有限数量的具有重要经济利益的病毒靶点，对于这些靶点，商业上标准化的检测方法是可用的。由于几个原因，尽管PCR测定是有利的，但它们在用于其他传染病的常规诊断中的实施效果不佳。结合核酸的自动化样品分离，实时PCR提供了一个理想的平台，用于开发具有临床意义的广泛感染因子的分子测定。由于其简便、快速、污染风险小等优点，实时荧光PCR技术将取代传统的PCR检测方法，在临床微生物学中得到广泛的应用。
PCR based assays are currently used routinely in most microbiology laboratories. But, with few exceptions, they are restricted to the field of virology, especially to a limited number of viral targets with important economical interest for which commercially well standardized assays are available. For several reasons, it has had a poor implementation of the PCR assays into routine diagnostics for other infectious diseases despite they are advantageous. Combined with automated sample isolation of nucleic acids, real-time PCR gives an ideal platform for the development of molecular assays for a wide range of infectious agents with clinical interest. Because of its advantages, as simplicity, rapidity and minor risk of contamination, real-time PCR will go replace conventional PCR assays and its use will extend to a wide range of applications in clinical microbiology.