Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma
Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma
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DOI：
10.1080/15321810801888480
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发表时间：
2008-01-01
影响因子：
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通讯作者：
Avanzi, Gian Carlo
中科院分区：
文献类型：
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作者：
Alciato, Federica;Sainaghi, Pier Paolo;Avanzi, Gian Carlo
Gas 6蛋白可能与人类疾病有关，但缺乏有效的血浆检测方法。因此，我们开发了一种夹心酶联免疫吸附测定（ELISA）方法，使用市售试剂。制备适当的基于血浆的基质以优化测定。ELISA方法显示批间和批内变异系数低于15%。回收率均在预期值的15%以内。61名健康供体的血浆Gas 6浓度为20.3 +/- 3.8 ng/mL。我们的检测方法符合FDA对生物分析方法验证的精密度和准确度的要求，适用于研究或诊断目的。
Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3 +/- 3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.