A honey bee Dscam family member, AbsCAM, is a brain-specific cell adhesion molecule with the neurite outgrowth activity which influences neuronal wiring during development
A honey bee Dscam family member, AbsCAM, is a brain-specific cell adhesion molecule with the neurite outgrowth activity which influences neuronal wiring during development
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DOI：
10.1111/j.1460-9568.2006.05270.x
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Kadowaki, Tatsuhiko
中科院分区：
文献类型：
--
作者：
Funada, Masahiro;Hara, Hiroaki;Kadowaki, Tatsuhiko
免疫球蛋白超家族（IgSF）已被证明在几种模式无脊椎动物（黑腹果蝇和秀丽隐杆线虫）中通过细胞间识别和通讯在神经系统的发育和维持中发挥作用。为了进一步探索IgSF在具有更复杂行为的无脊椎动物脑中的功能，我们从蜜蜂（Apis mellifera）中鉴定并表征了一种新的脑特异性Dscam家族成员AbsCAM。AbsCAM蛋白的水平在新孵化的蜜蜂中很高，并且随着年龄的增长而显著降低。AbsCAM蛋白水平在相同年龄的工蜂中是恒定的，这表明它主要由年龄而不是成年大脑中的任务决定。两种不同的AbsCAM转录本（AbsCAM-Ig 7 A和B）的编码免疫球蛋白结构域7的外显子11的选择性剪接产生的年龄依赖性的方式。AbsCAM表达于突触密度高的主要脑神经末梢。AbsCAM在体外可介导同种型特异性的嗜同性细胞粘附，并通过异位表达影响果蝇胚胎中枢神经系统和成年蘑菇体的轴突投射。此外，AbsCAM促进从蜜蜂蛹脑中分离的培养神经元的轴突生长。因此，这些结果表明，AbsCAM是第一个蜜蜂IgSF牵连在蜜蜂大脑发育过程中的神经元布线功能。
The immunoglobulin superfamily (IgSF) has been indicated as functioning in the development and maintenance of nervous systems through cell-cell recognition and communication in several model invertebrates, Drosophila melanogaster and Caenorhabditis elegans. To further explore the functions of the IgSF in the brain of an invertebrate with more complex behavior, we identified and characterized a novel brain-specific Dscam family member, AbsCAM, from honey bee (Apis mellifera). The level of the AbsCAM protein was high in newly hatched bees and was dramatically reduced with age. The AbsCAM protein level was constant among worker bees of the same age performing different tasks, suggesting that it was primarily determined by age and not task in adult brains. Two different AbsCAM transcripts (AbsCAM-Ig7A and B) were generated by the alternative splicing of exon 11 encoding immunoglobulin domain 7 in an age-dependent manner. AbsCAM was expressed in the major brain neuropils where the synaptic density was high. AbsCAM can mediate the isoform-specific homophilic cell adhesion in vitro, and affected the axonal projections in Drosophila embryonic central nervous system and adult mushroom body by ectopic expression. Furthermore, AbsCAM promoted the neurite outgrowth of cultured neurons isolated from honey bee pupal brains. These results thus suggest that AbsCAM is the first honey bee IgSF implicated as functioning in neuronal wiring during honey bee brain development.