Rabbits fed on β-carotene have higher serum levels of all-trans retinoic acid than those receiving no β-carotene
Rabbits fed on β-carotene have higher serum levels of all-trans retinoic acid than those receiving no β-carotene
复制标题
喂食 β-胡萝卜素的兔子比不喂食 β-胡萝卜素的兔子血清中全反式视黄酸水平更高
作者：
Y. Folman;R. Russell;G. Tang;G. Wolf
本工作的目的是确定递增剂量的β-胡萝卜素补充剂对兔血清维甲酸水平的影响。将7周龄雌性大耳白兔随机分为4组，分别饲喂含维生素A 1.72 mg/kg和不含β-胡萝卜素或β-胡萝卜素1、2、4 mg/kg/d的对照明胶颗粒饲料9周，分别于实验开始后3、6、9周采血，经双相萃取后用反相高效液相色谱法测定全反式维甲酸的浓度。联合对照组和1 mgβ-胡萝卜素/kg组大鼠第3、6、9周血清维甲酸平均浓度分别为3.80、3.06和2.40 nM。联合2、4 mgβ-胡萝卜素/kg组大鼠第3、6、9周的血清维甲酸浓度分别为4.80nM(P<0.005)、3.76nM(P<0.05)和4.90nM(P<；005)。SAS(SAS Institute Inc.，1985)一般线性模型重复测量方差分析显示，处理(P<；0.01)、时间(P<；0.05)和处理×时间交互作用(P<；0.05)的效果具有统计学意义。结论是，与不服用β-胡萝卜素的兔相比，服用β-胡萝卜素的兔血清中全反式维甲酸的浓度更高。
The aim of the present work was to determine the effect of giving increasing doses of supplements of β-carotene on serum retinoic acid levels in rabbits. Four groups of 7-week-old female rabbits were fed for 9 weeks on a pelleted diet containing 1.72 mg vitamin A as retinyl acetate/kg and including control gelatin beadlets devoid of β-carotene or 1, 2 or 4 mg β-carotene/kg body-weight per d. Serum was collected at 3, 6 and 9 weeks after the beginning of the experiment and the concentration of all-trans retinoic acid was determined by a gradient reverse-phase high-performance liquid chromatography system following a double-phase extraction. The average concentration of retinoic acid in serum of the combined control and 1 mg β-carotene/kg groups was 3.80, 3.06 and 2.40 nM at 3, 6 and 9 weeks respectively. The concentrations of retinoic acid in serum of the combined 2 and 4 mg β-carotene/kg groups were 4.80 nM (P < 0.05), 3.76 nM (not significant) and 4.90 nM (P < 0.005) at 3, 6 and 9 weeks respectively. A SAS (SAS Institute Inc., 1985) general linear model repeated-measures analysis of variance revealed that the effects of treatment (P <0.01), time (P < 0.05) and treatment x time interaction (P < 0.05) were statistically significant. It is concluded that giving β-carotene is associated with higher concentrations of all-trans retinoic acid in the serum of rabbits than in those receiving no β-carotene.
影响因子：
3.7
作者：
Bazrafshan E;Kord Mostafapour F;Farzadkia M;Ownagh KA;Mahvi AH
通讯作者：
Mahvi AH
从 β-胡萝卜素生物合成视黄酸。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Napoli,JL;Race,KR
通讯作者：
Race,KR
大鼠组织细胞质中细胞视黄醇和细胞视黄酸结合蛋白的放射免疫化学测定。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ong,DE;Crow,JA;Chytil,F
通讯作者：
Chytil,F