Comparison of Echinococcus multilocularis and Echinococcus granulosus hydatid fluid proteome provides molecular strategies for specialized host-parasite interactions.
Comparison of Echinococcus multilocularis and Echinococcus granulosus hydatid fluid proteome provides molecular strategies for specialized host-parasite interactions.
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DOI：
10.18632/oncotarget.20761
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发表时间：
2017-11-14
期刊：
影响因子：
--
通讯作者：
Kong Y
中科院分区：
文献类型：
--
作者：
Ahn CS;Kim JG;Han X;Kang I;Kong Y
泡型包虫病和囊型包虫病由多房棘球绦虫和细粒棘球绦虫的后鞭毛虫引起，在多个地区流行，并引起有害的人畜共患蠕虫硫酶。包虫体液含有寄生虫和宿主来源成分的蛋白质和非蛋白质分泌物，严重影响宿主-寄生虫的相互作用和疾病进展。我们对完全成熟的多房棘球绦虫囊泡(感染后9个月)和细粒棘球绦虫包囊(第2期)进行了HF蛋白质组图谱分析。我们在每种液体中分别鉴定出120种和153种蛋白质。56 84个蛋白质代表不同的物种，44个和66个是寄生虫，12个和18个是寄主来源的蛋白质。5种主要寄生虫蛋白群，包括抗原B亚型、代谢酶、蛋白酶和抑制物、细胞外基质分子(ECM)和发育蛋白，在这两种液体中大量分布，也仅在一种或另一种样品中分布。细粒棘球藻中碳水化合物代谢酶含量丰富。在多房棘球藻HF中，构成细胞外基质的蛋白质高度表达，这些蛋白质可能促进黏附和细胞发生。这些分子通过蛋白质-蛋白质相互作用网络具有物理和功能关系以及它们的生化特性。12种宿主来源的蛋白质主要被分离到血清成分中。这些HFs中常见和独特的主要蛋白质及其共生相互作用组关系可能反映了它们在宿主体内寄生虫的成熟、入侵和寿命的相似但不同的模式中所起的生物学作用。
Alveolar and cystic echinococcoses, caused by the metacestodes of Echinococcus multilocularis and E. granulosus, are prevalent in several regions and invoke deleterious zoonotic helminthiases. Hydatid fluid (HF), which contains proteinaceous and non-proteinaceous secretions of the parasite- and host-derived components, critically affects the host-parasite interplay and disease progression. We conducted HF proteome profiling of fully mature E. multilocularis vesicle (nine months postinfection) and E. granulosus cyst (stage 2). We identified 120 and 153 proteins, respectively, in each fluid. Fifty-six and 84 proteins represented distinct species; 44 and 66 were parasites, and 12 and 18 were host-derived proteins. The five major parasite protein populations, which included antigen B isoforms, metabolic enzymes, proteases and inhibitors, extracellular matrix molecules (ECMs), and developmental proteins, were abundantly distributed in both fluids and also exclusively in one sample or the other. Carbohydrate-metabolizing enzymes were enriched in E. granulosus HF. In the E. multilocularis HF, proteins that constitute ECMs, which might facilitate adhesion and cytogenesis, were highly expressed. Those molecules had physical and functional relationships along with their biochemical properties through protein-protein interaction networks. Twelve host-derived proteins were largely segregated to serum components. The major proteins commonly and uniquely detected in these HFs and their symbiotic interactome relationships might reflect their biological roles in similar but distinct modes of maturation, invasion, and the longevity of the parasites in the hosts.