The C3HC type zinc-finger protein (ZFC3) interacting with Lon/MAP1 is important for mitochondrial gene regulation, infection hypha development and longevity of Magnaporthe oryzae
The C3HC type zinc-finger protein (ZFC3) interacting with Lon/MAP1 is important for mitochondrial gene regulation, infection hypha development and longevity of Magnaporthe oryzae
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C3HC型锌指蛋白（ZFC3）与Lon/MAP1相互作用对于稻瘟病菌的线粒体基因调控、感染菌丝发育和寿命很重要
DOI：
10.1186/s12866-020-1711-4
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhang SHi-Hong
中科院分区：
文献类型：
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作者：
Liu SHaoshuai;Wei Yi;Zhang SHi-Hong
稻瘟病是由稻瘟病菌引起的一种典型的真菌病害，线粒体三磷酸腺苷依赖的Lon蛋白酶(MAP1)已被证明参与稻瘟病的发生。我们先前筛选到一个C3HC型锌指结构域蛋白(ZFC3)，它与MAP1相互作用。本研究旨在研究ZFC3蛋白INM的生物学功能。结果首次证实ZFC3-RFP融合蛋白定位于线粒体内。缺失突变株(∆ZFC3)的mtATP6，尤其是mtATP8的表达增强，而nATP9的表达几乎没有变化。ΔZFC3产生的分生孢子更多，对多种逆境的耐受性也更强。基因敲除菌株表现出更多的黑色素积累，表明对衰老的敏感性。结论ΔZFC3在基因调控、胁迫反应、细胞壁完整性、寿命、分生孢子形成、侵染菌丝发育和MAP1介导的致病力等方面发挥着重要的调控作用。米饭。
BackgroundThe rice blast is a typical fungal disease caused byMagnaporthe oryzae, and the mitochondrial ATP-dependent Lon protease (MAP1) has been proven to be involved in blast development. We previously screened a C3HC type Zinc-finger domain protein (ZFC3), which is interacted with MAP1. The purpose of this research was to study the biological function of ZFC3 protein inM. oryzae.ResultsWe first confirmed that the ZFC3-RFP fusion protein is localized within the mitochondria. The deleted mutant strains of ZFC3 (∆ZFC3) showed the enhanced expression level of mtATP6, particularly mtATP8, and almost unchanged nATP9. ΔZFC3produces more conidia and more tolerance to multiple stressors. The knock-out strain shows more melanin accumulation suggests the susceptibility to aging. ΔZFC3displays faster early-stage hypha infiltration involved in MAP1-mediated pathogenicity in host rice.ConclusionThese results support the view that ZFC3 is a key regulator involved in gene regulation, stress response, cell wall integrity, longevity, conidiation, infection hypha development and MAP1-mediated pathogenicity inM. oryzae.