Mercury-199 NMR of the metal receptor site in MerR and its protein-DNA complex.
Mercury-199 NMR of the metal receptor site in MerR and its protein-DNA complex.
复制标题
DOI：
10.1126/science.7716541
复制
发表时间：
1995-04
期刊：
影响因子：
56.9
通讯作者：
L. Utschig;J. Bryson;T. O’Halloran
中科院分区：
文献类型：
--
作者：
L. Utschig;J. Bryson;T. O’Halloran
汞-199核磁共振（NMR）已经提供了结构的见解到金属受体位点的MerR金属调节蛋白单独和在一个复杂的监管目标，DNA。一维和二维的NMR数据是一致的三角形平面汞-硫醇配位环境，仅由半胱氨酸侧链和解决结构方面的金属离子识别和变构机制。这些研究建立了199 Hg NMR技术作为有用的探针的金属配位环境的调节蛋白，铜酶，锌转录因子复合物高达50千道尔顿。
Structural insights have been provided by mercury-199 nuclear magnetic resonance (NMR) into the metal receptor site of the MerR metalloregulatory protein alone and in a complex with the regulatory target, DNA. The one- and two-dimensional NMR data are consistent with a trigonal planar Hg-thiolate coordination environment consisting only of Cys side chains and resolve structural aspects of both metal ion recognition and the allosteric mechanism. These studies establish 199Hg NMR techniques as useful probes of the metal coordination environment of regulatory proteins, copper enzymes, and zinc transcription factor complexes as large as 50 kilodaltons.