A major quantitative trait locus affecting resistance to Tilapia lake virus in farmed Nile tilapia (Oreochromis niloticus).
A major quantitative trait locus affecting resistance to Tilapia lake virus in farmed Nile tilapia (Oreochromis niloticus).
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DOI：
10.1038/s41437-021-00447-4
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发表时间：
2021-09
期刊：
影响因子：
3.8
通讯作者：
Houston RD
中科院分区：
文献类型：
--
作者：
Barría A;Trịnh TQ;Mahmuddin M;Peñaloza C;Papadopoulou A;Gervais O;Chadag VM;Benzie JAH;Houston RD
近年来，增强宿主对传染病的抵抗力作为农场动物育种计划的主要目标受到越来越多的关注。将现场数据与基因组工具相结合可以提供了解抗病性遗传结构的机会，从而为疾病控制带来新的机会。在当前的研究中，进行了一项全基因组关联研究，以评估对罗非鱼湖病毒（TiLV）的抵抗力，罗非鱼湖病毒是影响尼罗罗非鱼（Oreochromis niloticus）的最大威胁之一；全球重要的水产养殖品种。在 GIFT 品系的纯系群体中观察到池塘爆发 TiLV，其中 950 条鱼被分类为幸存者或死亡，并使用 65 K SNP 阵列进行基因分型。在Oni22染色体上鉴定出一个显着的大效应QTL。在最显着的 SNP（P 值 = 4.51E−10）处，抗性等位基因纯合的罗非鱼的平均死亡率为 11%，而易感等位基因纯合的罗非鱼的平均死亡率为 43%。在该 QTL 中鉴定出与宿主对病毒感染的反应相关的几个候选基因，包括 lgals17、vps52 和 trim29。这些结果提供了一个罕见的例子，说明主要 QTL 影响对养殖动物至关重要的性状。 QTL 区域的遗传标记在标记辅助选择方面具有潜力，可提高宿主的抗性，为目前几乎不存在其他控制或缓解方案的传染病提供遗传解决方案。
Enhancing host resistance to infectious disease has received increasing attention in recent years as a major goal of farm animal breeding programs. Combining field data with genomic tools can provide opportunities to understand the genetic architecture of disease resistance, leading to new opportunities for disease control. In the current study, a genome-wide association study was performed to assess resistance to the Tilapia lake virus (TiLV), one of the biggest threats affecting Nile tilapia (Oreochromis niloticus); a key aquaculture species globally. A pond outbreak of TiLV in a pedigreed population of the GIFT strain was observed, with 950 fish classified as either survivor or mortality, and genotyped using a 65 K SNP array. A significant QTL of large effect was identified on chromosome Oni22. The average mortality rate of tilapia homozygous for the resistance allele at the most significant SNP (P value = 4.51E−10) was 11%, compared to 43% for tilapia homozygous for the susceptibility allele. Several candidate genes related to host response to viral infection were identified within this QTL, including lgals17, vps52, and trim29. These results provide a rare example of a major QTL affecting a trait of major importance to a farmed animal. Genetic markers from the QTL region have potential in marker-assisted selection to improve host resistance, providing a genetic solution to an infectious disease where few other control or mitigation options currently exist.
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DOI：
10.1534/g3.118.200053
发表时间：
2018-03-28
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Barría A;Christensen KA;Yoshida GM;Correa K;Jedlicki A;Lhorente JP;Davidson WS;Yáñez JM
通讯作者：
Yáñez JM
DOI：
10.1534/g3.117.041780
发表时间：
2017-07-05
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Gutierrez AP;Turner F;Gharbi K;Talbot R;Lowe NR;Peñaloza C;McCullough M;Prodöhl PA;Bean TP;Houston RD
通讯作者：
Houston RD
影响因子：
4.4
作者：
Houston RD;Taggart JB;Cézard T;Bekaert M;Lowe NR;Downing A;Talbot R;Bishop SC;Archibald AL;Bron JE;Penman DJ;Davassi A;Brew F;Tinch AE;Gharbi K;Hamilton A
通讯作者：
Hamilton A
影响因子：
4.5
作者：
Ha Thanh Dong;Senapin, Saengchan;Mohan, Chadag Vishnumurthy
通讯作者：
Mohan, Chadag Vishnumurthy
影响因子：
9.2
作者：
Conte, Matthew A.;Joshi, Rajesh;Kocher, Thomas D.
通讯作者：
Kocher, Thomas D.