Further characterization of low density mononuclear cells: FACS-assisted analysis of human MLR stimulators.
Further characterization of low density mononuclear cells: FACS-assisted analysis of human MLR stimulators.
复制标题
低密度单核细胞的进一步表征：人类 MLR 刺激物的 FACS 辅助分析。
DOI：
10.4049/jimmunol.134.2.779
复制
发表时间：
1985
影响因子：
4.4
通讯作者：
D. Horwitz
中科院分区：
文献类型：
--
作者：
F. Santiago;A. Bakke;J. Woodward;R. O’Brien;D. Horwitz
以前，我们报道了所有的同种异体混合白细胞反应（MLR）的强有力的刺激剂包含在一个异质性的低密度部分的人外周血单核细胞（PBM）细胞。我们进一步的特点是人类MLR刺激物染色，他们与高度特异性的单克隆抗体，然后分析和分离它们与荧光激活细胞分选仪。这些研究揭示了两个群体的低密度细胞具有强有力的同种异体刺激活性。一个群体是与抗OKM 1、MO.2反应并表达C3 b以及Fc-IgG受体的单核细胞亚群。第二群体表现出甚至更大的刺激能力，并且不表达任何这些单核细胞标志物。此外，这些细胞不是吞噬细胞，不与α-萘基酯酶反应。它们包含约10%的低密度部分或0.5%的PBM。这些细胞最有可能是淋巴树突细胞，在各种物种中被描述为有效的MLR刺激物。与同种异体MLR相反，只有表现出树突状细胞特征的低密度细胞类型诱导了有效的自体MLR。
Previously, we reported that all of the potent stimulators of the allogeneic mixed leukocyte reaction (MLR) are contained in a heterogeneous low density fraction of human peripheral blood mononuclear (PBM) cells. We have further characterized human MLR stimulators by staining them with highly specific monoclonal antibodies, and then analyzing and separating them with the fluorescence-activated cell sorter. These studies revealed two populations of low density cells with potent allogeneic stimulatory activity. One population is a monocyte subset that reacts with anti-OKM1, MO.2, and expresses C3b as well as Fc-IgG receptors. The second population exhibits even greater stimulatory capacity and does not express any of these monocyte markers. Moreover, these cells are not phagocytic and do not react with alpha-naphthyl esterase. They comprise approximately 10% of the low density fraction or 0.5% of PBM. These cells are most likely lymphoid dendritic cells, described in various species as potent MLR stimulators. In contrast to the allogeneic MLR, only the low density cell type exhibiting dendritic cell characteristics induced a potent autologous MLR.