High-performance liquid chromatographic separation of phospholipid precursors and thier direct measurement by automatic phosphorus analysis
High-performance liquid chromatographic separation of phospholipid precursors and thier direct measurement by automatic phosphorus analysis
复制标题
磷脂前体的高效液相色谱分离及其自动磷分析直接测量
DOI：
10.1016/s0021-9673(00)82543-1
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发表时间：
1981
影响因子：
4.1
通讯作者：
S. Bessman
中科院分区：
文献类型：
--
作者：
J. Kaitaranta;P. Geiger;S. Bessman
在这份报告中，我们描述了一种改进的方法，用于分析生物样品中磷脂合成或分解的磷酸化代谢中间体。高效液相色谱的应用使得最常见的代谢物能够在不到1.5小时内分离，并且与自动磷分析仪的直接连接使得同时定量成为可能。该方法目前正应用于动物和人体组织中代谢差异的研究。
In this report we have described an improved method for the analysis of phosphorylated metabolic intermediates of the phospholipid synthesis or breakdown in biological samples. The application of high-performance liquid chromatography enables the separation of the most common metabolites within less than 1.5 h, and the direct connection to the automatic phosphorus analyzer makes a simultaneous quantiation possibl. The method is currently being applied to studies of metabolic differences in animal and human tissues.