The 20-pS chloride channel of the human airway epithelium.
The 20-pS chloride channel of the human airway epithelium.
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人类气道上皮的 20-pS 氯离子通道。
DOI：
10.1016/s0006-3495(90)82525-x
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发表时间：
1990
影响因子：
3.4
通讯作者：
S. F. Man
中科院分区：
文献类型：
--
作者：
M. Duszyk;Andrew S. French;S. F. Man
采用单通道内面向外膜片钳技术对原代培养的人气道上皮细胞顶膜氯离子通道进行了研究。从单个分离的细胞或从汇合组边缘的细胞获得贴片。最常见的通道，在24%的所有补丁，有一个电导约20 pS，并在对称的氯化物溶液中的线性电流-电压关系。通道对阴离子的选择性顺序为NO3-> Cl-> HCO 3-，对葡萄糖酸根离子不透过，表明通道直径在4.7 ~ 5.5之间。通道电流在高浓度氯离子时达到饱和，通道电流与氯离子浓度之间的关系可用Michaelis-Menten方程近似。通道的阴离子渗透性及其电流与浓度关系的分析表明，它可以用单离子通道理论来描述，通道内具有相对较弱的结合位点。通道开放和关闭的持续时间的直方图使用对数分箱技术构建，可以很好地拟合三重指数分布，这表明通道至少有三个开放和三个关闭状态。
The single-channel inside-out patch clamp technique was used to characterize chloride channels in the apical membranes of human airway epithelial cells maintained in primary culture. Patches were obtained from single isolated cells or from cells at the edges of confluent groups. The channel seen most often, in 24% of all patches, had a conductance of approximately 20 pS and had a linear current-voltage relationship in symmetric chloride solutions. The anion selectivity sequence for the channel was NO3- greater than Cl- greater than HCO3-, and it was impermeable to gluconate ions, indicating that the channel diameter lies between 4.7 and 5.5 A. Current through the channel saturated at high chloride concentrations, and the relationship between channel current and chloride concentration could be approximated by the Michaelis-Menten equation. Analysis of the channel's anion permeability and its current vs. concentration relationship indicates that it can be described by the one-ion channel theory, with a relatively weak binding site inside the channel. Histograms of channel open and closed durations were constructed using the log binning technique and could be well fitted by triple exponential distributions, suggesting that the channel has at least three open and three closed states.
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通过气道上皮细胞输送电解质。
影响因子：
33.6
作者：
Welsh,MJ
通讯作者：
Welsh,MJ
DOI：
--
发表时间：
1986-11
期刊：
Federation proceedings
影响因子：
--
作者：
R. Frizzell;D. Halm;G. Rechkemmer;R. Shoemaker
通讯作者：
R. Frizzell;D. Halm;G. Rechkemmer;R. Shoemaker
DOI：
10.1126/science.2424085
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Welsh,MJ
通讯作者：
Welsh,MJ
人鼻上皮细胞在培养物中的生长和分化。
DOI：
10.1164/arrd.1985.132.2.311
发表时间：
1985
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Wu,R;Yankaskas,J;Cheng,E;Knowles,MR;Boucher,R
通讯作者：
Boucher,R
囊性纤维化上皮细胞中的氯离子通道调节。
DOI：
--
发表时间：
1987
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
Frizzell,RA;Schoumacher,RA;Halm,DR
通讯作者：
Halm,DR