Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes.
Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes.
复制标题
旧小鼠卵母细胞生殖老化的多光谱自动荧光特征。
DOI：
10.1007/s10522-022-09957-y
复制
发表时间：
2022-04
期刊：
影响因子：
4.5
通讯作者：
Goldys, Ewa M.
中科院分区：
文献类型：
--
作者：
Campbell, Jared M.;Mahbub, Saabah B.;Bertoldo, Michael J.;Habibalahi, Abbas;Goss, Dale M.;Ledger, William L.;Gilchrist, Robert B.;Wu, Lindsay E.;Goldys, Ewa M.
年龄的增长对女性生育率产生重大不利影响，随着人口老龄化，这会产生重大的社会影响。这种影响主要是通过卵母细胞质量的下降来介导的。随着生物学年龄的增长，卵母细胞质量下降是女性生育能力的最大限速因素。在这里，我们使用了无标记，非侵入性的多光谱成像来识别年轻和老年动物卵母细胞独特的自发荧光谱。判别分析表明，年轻的卵母细胞有一个独特的自发荧光的轮廓，准确地区分他们从老年卵母细胞。我们最近表明，用烟酰胺腺嘌呤二核苷酸（NAD+）前体烟酰胺单核苷酸（NMN）治疗恢复了老年动物的卵母细胞质量和生育力，当我们的分析应用于用NMN治疗的老年动物的卵母细胞时，这些卵母细胞中有85%被归类为具有年轻动物的自体荧光特征。使用稳健依赖成分分析（RoDECA）算法进行的光谱解混表明，NMN处理改变了卵母细胞的代谢特征，增加了游离NAD（P）H、蛋白结合NAD（P）H、氧化还原比以及结合与游离NAD（P）H的比率。在用NMN处理的小鼠中，同时具有高NAD（P）H和黄素含量的卵母细胞的频率也显著增加。年轻和老年+ NMN卵母细胞有一个更平滑的光谱分布，与年轻卵母细胞的NAD（P）H的分布特别不同于老年卵母细胞。识别老化过程中卵母细胞自发荧光的多光谱谱可以作为卵母细胞质量的非侵入性和灵敏的测量方法。
Increasing age has a major detrimental impact on female fertility, which, with an ageing population, has major sociological implications. This impact is primarily mediated through deteriorating quality of the oocyte. Deteriorating oocyte quality with biological age is the greatest rate-limiting factor to female fertility. Here we have used label-free, non-invasive multi-spectral imaging to identify unique autofluorescence profiles of oocytes from young and aged animals. Discriminant analysis demonstrated that young oocytes have a distinct autofluorescent profile which accurately distinguishes them from aged oocytes. We recently showed that treatment with the nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide mononucleotide (NMN) restored oocyte quality and fertility in aged animals, and when our analysis was applied to oocytes from aged animals treated with NMN, 85% of these oocytes were classified as having the autofluorescent signature of young animals. Spectral unmixing using the Robust Dependent Component Analysis (RoDECA) algorithm demonstrated that NMN treatment altered the metabolic profile of oocytes, increasing free NAD(P)H, protein bound NAD(P)H, redox ratio and the ratio of bound to free NAD(P)H. The frequency of oocytes with simultaneously high NAD(P)H and flavin content was also significantly increased in mice treated with NMN. Young and Aged + NMN oocytes had a smoother spectral distribution, with the distribution of NAD(P)H in young oocytes specifically differing from that of aged oocytes. Identifying the multispectral profile of oocyte autofluorescence during aging could have utility as a non-invasive and sensitive measure of oocyte quality.
登录
查看更多内容
影响因子：
6.4
作者：
Habibalahi, Abbas;Bala, Chandra;Goldys, Ewa M.
通讯作者：
Goldys, Ewa M.
影响因子：
4.6
作者：
Gosnell ME;Anwer AG;Mahbub SB;Menon Perinchery S;Inglis DW;Adhikary PP;Jazayeri JA;Cahill MA;Saad S;Pollock CA;Sutton-McDowall ML;Thompson JG;Goldys EM
通讯作者：
Goldys EM
影响因子：
3
作者：
Duong, Haison;Han, Martin
通讯作者：
Han, Martin
DOI：
10.5653/cerm.2012.39.2.46
发表时间：
2012-06
期刊：
Clinical and experimental reproductive medicine
影响因子：
--
作者：
Lee S;Song JY;Ku SY;Kim SH;Kim T
通讯作者：
Kim T
影响因子：
4.6
作者：
Abd El Aziz M;Selim IM;Xiong S
通讯作者：
Xiong S