UPTAKE AND DEGRADATION OF FILAMENTOUS ACTIN AND VITAMIN-D-BINDING PROTEIN IN THE RAT
UPTAKE AND DEGRADATION OF FILAMENTOUS ACTIN AND VITAMIN-D-BINDING PROTEIN IN THE RAT
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DOI：
10.1042/bj2740237
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发表时间：
1991-02-15
影响因子：
4.1
通讯作者：
DREVON, CA
中科院分区：
文献类型：
--
作者：
DUELAND, S;NENSETER, MS;DREVON, CA
本文研究了碘-125-酪胺-纤维二糖标记的丝状肌动蛋白、维生素D结合蛋白（DBP）和肌动蛋白-DBP复合物在大鼠体内的摄取和降解。 肌动蛋白和肌动蛋白-DBP复合物从血浆中清除的速度比DBP快。 在给药后10至30分钟之间，约40%的注射肌动蛋白在肝脏中被回收。 当静脉注射标记的肌动蛋白或DBP-肌动蛋白复合物时，在肝实质和内皮细胞中分别检测到约35%和40%的总放射性。 当仅注射标记的DBP时，约肝脏中回收的放射性的55%在枯否细胞中。 这些结果表明，肌动蛋白靶向DBP-肌动蛋白复合物的内皮细胞和肝实质细胞。 细丝肌动蛋白也采取了大量的，并在一个快速的速度在实质以及非实质肝细胞在体外。 我们的数据表明，大鼠有一种机制，以清除肌动蛋白和DBP-肌动蛋白复合物从血浆和实质和非实质肝细胞都参与了这一过程。
Tissue uptake and degradation of I-125-tyramine-cellobiose-labelled filamentous actin, vitamin D-binding protein (DBP) and actin-DBP complex were studied in the rat. Actin and actin-DBP complex were cleared from plasma at a faster rate than was DBP. About 40% of injected actin was recovered in the liver between 10 and 30 min after administration. Of the total radioactivity recovered in the liver, about 35% and 40% was detected in parenchymal and endothelial cells respectively when labelled actin or DBP-actin complex was injected intravenously. When labelled DBP alone was injected, approx. 55% of the radioactivity recovered in liver was in the Kupffer cells. These results suggest that actin is targeting the DBP-actin complex to the endothelial and parenchymal liver cells. Filamentous actin was also taken up in large amounts and at a rapid rate in parenchymal as well as non-parenchymal liver cells in vitro. Our data indicate that the rat has a mechanism to clear actin and the DBP-actin complex from plasma and that both parenchymal and non-parenchymal liver cells are involved in this process.