AP-2β inhibits hepatocellular carcinoma invasion and metastasis through Slug and Snail to suppress epithelial-mesenchymal transition.
AP-2β inhibits hepatocellular carcinoma invasion and metastasis through Slug and Snail to suppress epithelial-mesenchymal transition.
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AP-2β通过Slug和Snail抑制肝细胞癌侵袭和转移抑制上皮间质转化
DOI：
10.7150/thno.25166
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发表时间：
2018
期刊：
影响因子：
12.4
通讯作者：
Xiang S
中科院分区：
文献类型：
--
作者：
Yang L;Qiu J;Xiao Y;Hu X;Liu Q;Chen L;Huang W;Li X;Li L;Zhang J;Ding X;Xiang S
Transcription factor AP-2β plays an important role in human cancer, but its clinical significance in hepatocellular carcinogenesis is largely unknown. Methods: AP-2β expression was detected in human hepatocellular cancer (HCC) tissues and cell lines. The effects of AP-2β on HCC proliferation, migration, invasion, tumor formation and metastasis were evaluated by MTT, colony formation and transwell assays in vitro and mouse experiments in vivo. The association between AP-2β and miR-27a/EMT markers in HCC cell lines and tissues was analyzed. Results: AP-2β expression was decreased in HCC tissues and cell lines. Reduced expression of AP-2β was significantly associated with more advanced tumor stages and larger tumor sizes. The overexpression of AP-2β reduced HCC proliferation, migration, invasion, tumor formation and metastasis in vitro and in vivo. Additionally, AP-2β overexpression increased the sensitivity of HCC cells to cisplatin. Moreover, AP-2β modulates the levels of EMT markers through Slug and Snail in HCC cell lines and tissues. Furthermore, oncogenic miR-27a inhibits AP-2β expression by binding to the AP-2β 3′ untranslated region (UTR) and reverses the tumor suppressive role of AP-2β. Conclusion: These results suggested that AP-2β is lowly expressed in HCC by inhibiting EMT signaling to regulate HCC cell growth and migration. Therefore, AP-2β in the novel miR-27a/AP-2β/Slug/EMT regulatory axis enhances the chemotherapeutic drug sensitivity of HCC and might represent a potential target for evaluating the treatment and prognosis of human HCC.
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影响因子：
3.7
作者：
Li X;Jiang J;Shi S;Bligh SW;Li Y;Jiang Y;Huang D;Ke Y;Wang S
通讯作者：
Wang S
影响因子：
4.7
作者：
Beger, M;Butz, K;Hoppe-Seyler, F
通讯作者：
Hoppe-Seyler, F
影响因子：
158.5
作者：
Llovet, Josep M.;Ricci, Sergio;Bruix, Jordi
通讯作者：
Bruix, Jordi
影响因子：
4.9
作者：
Fuke, T.;Yoshizaki, T.;Maegawa, H.
通讯作者：
Maegawa, H.
影响因子：
3.5
作者：
Fischer, U;Heckel, D;Meese, E
通讯作者：
Meese, E