Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction
Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction
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DOI：
10.1016/j.jbiosc.2013.02.011
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发表时间：
2013-08-01
影响因子：
2.8
通讯作者：
Park, Yongdoo
中科院分区：
文献类型：
--
作者：
Hong, Soyoung;Song, Seung-Joon;Park, Yongdoo
在三维（3D）基质内制造图案化微结构是组织工程和再生医学中具有挑战性的课题。开发了一种3D自由移动生物打印系统，并通过改变z轴移动速度和喷射速度的工艺参数来图案化水凝胶。在3D基质胶中的基于水凝胶的微纤维的图案化以4.5mm长和79至200 μ m宽的尺寸实现。使用3D移动轴生物打印机对与成纤维细胞（L929）、小鼠内皮细胞（MS 1）或人间充质干细胞（hMSC）混合的基于海曲康聚糖的水凝胶进行图案化，并在培养物中监测细胞行为长达16天。图案化水凝胶中的L929和MS 1细胞和hMSC揭示了细胞间的相互作用以及对细胞类型的形态依赖性。HMSCs通过细胞聚集形成球体，而L929细胞的细胞质量增加而无细胞聚集，MS 1分散到基质中而不是聚集。Rho激酶（ROCK）抑制剂和钙粘蛋白抗体可抑制hMSCs的聚集。这反映了细胞聚集和迁移与RhoA和细胞-细胞粘附分子之间的密切关系。血管生成特异性基因表达谱显示，与对照组相比，ROCK抑制剂组中CD 105的表达降低至22%。这些结果表明，3D基质中基于细胞的图案高度依赖于细胞聚集和随时间的迁移。(c)2013年，生物技术学会，日本。All rights reserved.
The fabrication of patterned microstructures within three-dimensional (3D) matrices is a challenging subject in tissue engineering and regenerative medicine. A 3D, free-moving bioprinting system was developed and hydrogels were patterned by varying the process parameters of z-axis moving velocity and ejection velocity. The patterning of hydrogel based microfibers in a 3D matrigel was achieved with dimensions of 4.5 mm length and widths from 79 to 200 mu m. Hyaluronan-based hydrogels mixed with fibroblasts (L929), mouse endothelial cells (MS1), or human mesenchymal stem cells (hMSCs) were patterned using a 3D moving axis bioprinter and cell behavior was monitored in culture for up to 16 days. L929 and MS1 cells and hMSCs in patterned hydrogel revealed cell-cell interactions and a morphological dependency on cell types. HMSCs formed spheres through cell aggregation, while L929 cells increased in cellular mass without cell aggregation and MS1 dispersed into the matrix instead of aggregating. The aggregation of hMSCs was attenuated by treatment with Rho kinase (ROCK) inhibitor and cadherin antibody. This reflected the close relationship between cell aggregation and migration with RhoA and cell-cell adhesion molecules. Angiogenic-specific gene expression profiles showed that expression of CD105 decreased to 22% in the ROCK inhibitor group compared to control group. These results showed that cell-based patterns in a 3D matrix are highly dependent on both cell aggregation and migration over time. (c) 2013, The Society for Biotechnology, Japan. All rights reserved.