Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor
Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor
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DOI：
10.1016/j.electacta.2012.02.011
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发表时间：
2012-04
影响因子：
6.6
通讯作者：
Cuicui Qiu;Xia Wang;Xueying Liu;Shifeng Hou;Houyi Ma
中科院分区：
文献类型：
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作者：
Cuicui Qiu;Xia Wang;Xueying Liu;Shifeng Hou;Houyi Ma
采用简单的物理吸附方法将葡萄糖氧化酶（GOx）稳定地固定在电沉积和电替代技术制备的纳米结构Au薄膜上，为制备形貌可控的Au薄膜提供了一种简便的方法，也为酶修饰电极的制备和应用提供了便利。所制备的酶电极（表示为GOx/Au/GCE）的一个明显优点是纳米Au膜为GOx提供了良好的微环境，并促进了GOx活性中心与电极之间的电子转移。循环伏安法（CV）结果表明，固定化氧化石墨烯在磷酸盐缓冲液中发生了直接、可逆、表面受限的氧化还原反应。在- 0.55V电压下，酶修饰电极在2.5 ~ 32.5μ moll−1和60 ~ 130μ moll−1范围内呈线性关系，检出限为0.32μmolL−1（S/N=3）。由于优异的稳定性、灵敏度和抗干扰能力，金薄膜在构建葡萄糖生物传感器方面具有广阔的应用前景。
Glucose oxidase (GOx) was stably immobilized via a simple physical adsorption method onto the nanostructured Au thin films fabricated by using electrodeposition and galvanic replacement technology, which provides a facile method to prepare morphology-controllable Au films and also facilitates the preparation and application of enzyme modified electrodes. An obvious advantage of the as-prepared enzyme electrode (denoted as GOx/Au/GCE) is that the nano-Au films provide a favorable microenvironment for GOx and facilitate the electron transfer between the active center of GOx and electrodes. Cyclic voltammetry (CV) results indicate that the immobilized GOx displayed a direct, reversible and surface-confined redox reaction in the phosphate buffer solution. Furthermore, the enzyme modified electrode was used as a glucose bioelectrochemical sensor, exhibiting a linear relationship in the concentration ranges of 2.5–32.5μmolL−1and 60–130μmolL−1with a detection limit of 0.32μmolL−1(S/N=3) at an applied potential of −0.55V. Due to the excellent stability, sensitivity and anti-interference ability, the Au thin films are hopeful in the construction of glucose biosensors.