BLEB FORMATION IN HEPATOCYTES DURING DRUG-METABOLISM IS CAUSED BY DISTURBANCES IN THIOL AND CALCIUM-ION HOMEOSTASIS
BLEB FORMATION IN HEPATOCYTES DURING DRUG-METABOLISM IS CAUSED BY DISTURBANCES IN THIOL AND CALCIUM-ION HOMEOSTASIS
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DOI：
10.1126/science.7112127
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
SMITH, MT
中科院分区：
文献类型：
--
作者：
JEWELL, SA;BELLOMO, G;SMITH, MT
多种有毒化学物质会导致分离的[大鼠]肝细胞质膜起泡。这些表面结构的改变在细胞死亡之前很久就已发生。起泡的形成似乎与线粒体外钙离子浓度的变化直接相关。这些变化可能降低了肝细胞骨架维持正常表面形态的能力。可溶性硫醇（尤其是谷胱甘肽）的浓度似乎调节着线粒体外钙离子库的大小。因此，细胞内硫醇和钙稳态的紊乱似乎是分离的肝细胞在毒性损伤过程中观察到的表面起泡的原因。
A wide variety of toxic chemicals cause blebbing of the plasma membrane in isolated [rat] hepatocytes. These alterations in surface structure occur well before cell death. The formation of blebs appears to be directly related to changes in the concentration of extramitochondrial Ca. These changes probably reduce the ability of the hepatocyte cytoskeleton to maintain normal surface morphology. The concentration of soluble thiols, notably glutathione, appears to regulate the size of the extramitochondrial Ca pool. Disturbances in intracellular thiol and Ca homeostasis therefore seem to be responsible for the surface blebbing observed during toxic injury to isolated hepatocytes.