Nutritional immunomodulation enhances cardiac allograft survival in rats treated with donor-specific transfusion and cyclosporine
Nutritional immunomodulation enhances cardiac allograft survival in rats treated with donor-specific transfusion and cyclosporine
复制标题
营养免疫调节可提高接受供者特异性输血和环孢素治疗的大鼠同种异体心脏移植物的存活率
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Alexander Jw
中科院分区：
文献类型：
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作者：
Levy Ae;Alexander Jw
本研究的目的是评估精氨酸、RNA和鱼油强化的肠内饮食（Impact）单独使用和与环孢素（CsA）和供体特异性输血（DST）联合使用对ACI：刘易斯大鼠心脏移植模型中同种异体移植物存活的疗效。动物自由采食标准大鼠饲料或Impact饲料。研究了六组;这些动物包括：未处理的接受者，喂食标准饮食或Impact饮食;接受者在移植前一天（第-1天）用CsA 10 mg/kg处理，然后在第0天->第+13天用2.5 mg/kg/d处理，并喂食标准饮食或Impact饮食;和动物在第-1天给予DST（1 ml），如前所述给予CsA，并喂食标准饮食或Impact饮食。未处理的动物标准饮食（第1组，n = 8）在7.0 +/- 0.0天时排斥其同种异体移植物，而那些喂食Impact的动物（第2组，n = 9）的移植物存活率为12.8 +/- 2.1天（P = 0.01 vs第1组）。仅用CsA和标准饮食治疗的动物（第3组，n = 9）在30.3 +/- 4.8天被拒绝，而CsA和Impact饮食的组合（第4组，n = 8）在33.0 +/- 9.5天被拒绝-与第3组相比，生存率略有改善。用DST/CsA和标准饮食处理的动物（第5组，n = 7）在72.1 +/-6.8天时排斥，而用Impact替代标准饮食（第6组，n = 8）导致移植物显著延长至275 +/-53天，n = 8（P < .015对比第1-5组）。这些数据表明，单独的Impact饮食可以具有有效的免疫调节特性，但可能需要添加DST/CsA才能实现其潜力。这些发现强调了饮食免疫调节疗法在预防排斥反应和促进实体器官移植耐受方面的潜力。
The aim of this study was to assess the efficacy of an enteral diet fortified with arginine, RNA, and fish oil (Impact), alone and in combination with cyclosporine (CsA) and donor-specific transfusion (DST) on allograft survival in the ACI:Lewis rat cardiac transplant model. Animals were fed ad libitum with either standard rat chow or Impact diet. Six groups were studied; these consisted of untreated recipients fed either standard diet or Impact diet; recipients treated with CsA 10 mg/kg on the day prior to engraftment (day-1) followed by 2.5 mg/kg/d, day 0-->day+13 and fed with either standard diet or Impact; and animals given a DST (1 ml) on day-1, CsA as described previously and fed either standard diet or Impact. Untreated animals standard diet (group 1, n = 8) rejected their allografts at 7.0 +/- 0.0 days, while those fed Impact (group 2, n = 9) had graft survival of 12.8 +/- 2.1 days, (P = .01 versus group 1). Animals treated with CsA alone and standard diet (group 3, n = 9) rejected at 30.3 +/- 4.8 days, while the combination of CsA and Impact diet (group 4, n = 8) rejected at 33.0 +/- 9.5 days--minimally improved survival compared with group 3. Animals treated with DST/CsA and standard diet (group 5, n = 7) rejected at 72.1 +/- 6.8 days, while the substitution of Impact for standard diet (group 6, n = 8) led to a significant graft prolongation to 275 +/- 53 days, n = 8 (P < .015 vs. groups 1-5). These data suggest that Impact diet alone can have potent immunomodulatory properties but may require the addition of DST/CsA to realize its potential. These findings underscore the potential of dietary immunomodulatory therapy to prevent rejection and promote tolerance to solid organ allografts.