Quantitative trait loci for yellow rust resistance in spring wheat doubled haploid populations developed from the German Federal ex situ genebank genetic resources
Quantitative trait loci for yellow rust resistance in spring wheat doubled haploid populations developed from the German Federal ex situ genebank genetic resources
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DOI：
10.1002/tpg2.20142
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发表时间：
2021-09-09
期刊：
影响因子：
4.2
通讯作者：
Roeder, Marion S.
中科院分区：
文献类型：
--
作者：
Draz, Ibrahim S.;Serfling, Albrecht;Roeder, Marion S.
小麦条锈菌的新抗源。小麦条锈病，其引起小麦（Triticum aestivum L.）中的一种广泛的毁灭性叶部病害，有需求。在这里，我们测试了两个双单倍体（DH）春小麦群体来自遗传资源的抗条锈病田间试验在德国和埃及。此外，我们还进行了全阶段抗性（幼苗抗性）试验。我们基于15 k Infinium SNP芯片基因分型数据进行了连锁作图，分别为DH群体1（103种基因型）和DH群体2（148种基因型）产生了3，567和3，457个多态性标记。在DH群体1中，我们确定了一个主要的和一致的数量性状位点（QTL）染色体1B上，解释了高达28%和39%的表型变异，在田间和幼苗测试，分别。有利等位基因由来自伊朗的地方品种'TRI-5645'贡献，并且最可能是抗条锈病（Yr）基因Yr 10。在DH群体2中，6 B染色体上的一个主效QTL的有利等位基因由代表法国品种‘Eureke’的品系‘TRI-5310’贡献。该QTL主要在德国环境中有效，解释了高达36%的表型变异。然而，在埃及，只有一个中度抗性QTL被确定在田间试验中，没有抗性QTL观察到在幼苗测试。我们的研究结果表明，遗传资源的有用性，以确定新的抗锈病来源，包括“勇士”的比赛PstS 10。
Novel resistance sources to the pathogen Puccinia striiformis f. sp. tritici, which causes yellow rust (stripe rust), a widespread devastating foliar disease in wheat (Triticum aestivum L.), are in demand. Here, we tested two doubled haploid (DH) spring wheat populations derived from the genetic resources for resistance to yellow rust in field trials in Germany and Egypt. Additionally, we performed tests for allstage resistance (seedling resistance). We performed linkage mapping based on 15k Infinium SNP chip genotyping data that resulted in 3,567 and 3,457 polymorphic markers for DH Population 1 (103 genotypes) and DH Population 2 (148 genotypes), respectively. In DH Population 1, we identified a major and consistent quantitative trait locus (QTL) on chromosome 1B that explained up to 28 and 39% of the phenotypic variation in the field and seedling tests, respectively. The favorable allele was contributed by the line 'TRI-5645', a landrace from Iran, and is most probably the yellow rust resistance (Yr) gene Yr10. In DH Population 2, the favorable allele of a major QTL on chromosome 6B was contributed by the line 'TRI-5310', representing the variety 'Eureke' from France. This QTL was mainly effective in the German environments and explained up to 36% of the phenotypic variation. In Egypt, however, only a moderate resistance QTL was identified in the field tests and no resistance QTL was observed in the seedling tests. Our results demonstrate the usefulness of genetic resources to identify novel sources of resistance to yellow rust, including the "Warrior" race PstS10.