Transfer of a dwarf gene from Brassica rapa to oilseed B-Napus, effects on agronomic traits, and development of a 'perfect' marker for selection
Transfer of a dwarf gene from Brassica rapa to oilseed B-Napus, effects on agronomic traits, and development of a 'perfect' marker for selection
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DOI：
10.1007/s11032-005-3734-9
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发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Osborn, TC
中科院分区：
文献类型：
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作者：
Muangprom, A;Mauriera, I;Osborn, TC
倒伏对于油菜甘蓝型油菜来说是一个严重的问题。矮化基因已被用来减少其他作物的倒伏，它们在小麦和水稻中的使用是“绿色革命”成功的主要因素。我们之前报道了白菜的单一、半显性、赤霉素不敏感矮化突变体（Brrga1-d），并提供了该基因与小麦“绿色革命”矮化基因同源性的证据。在本文中，我们报道了利用白菜和甘蓝的种间杂交和胚胎拯救来重新合成含有Brrga1-d矮化基因的甘蓝型油菜。矮化基因与商业杂交组合的两个亲本回交，并在田间实验中作为近交系和杂交系进行评估。即使在杂合基因型中以单一剂量存在时，Brrga1-d 基因也会降低甘蓝型油菜自交系和杂交系中的株高和倒伏。与接近等基因的野生型自交系相比，Brrga1-d 纯合自交系的种子产量降低，这可能是由于开花时间稍微延迟引起的昆虫损害。然而，Brrga1-d 纯合或杂合杂交体的产量与近等基因野生型杂交体的产量相似。此外，我们报告了一种用于选择 Brrga1-d 基因的“完美”PCR 标记，该标记基于检测导致矮化表型的核苷酸突变。
Lodging can be a serious problem in oilseed Brassica napus. Dwarf genes have been used to reduce lodging in other crops, and their use in wheat and rice was a major factor in the success of the 'Green Revolution'. We previously reported on a single, semi-dominant, gibberellin insensitive dwarf mutant of B. rapa (Brrga1-d), and provided evidence for homology of this gene to the wheat 'Green Revolution' dwarf gene. In this paper, we report using interspecific hybridization of B. rapa and B. oleracea and embryo rescue to resynthesize B. napus containing the Brrga1-d dwarf gene. The dwarf gene was backcrossed into two parents of a commercial hybrid combination and evaluated as inbred and hybrid lines in field experiments. The Brrga1-d gene reduced plant height and lodging in inbred and hybrid lines of B. napus, even when present as a single dose in heterozygous genotypes. Seed yields of inbred lines homozygous for Brrga1-d were reduced compared to near isogenic wild-type inbreds, presumably due to damage by insects caused by a slight delay in flowering time. However, yields of hybrids that were homozygous or heterozygous for Brrga1-d were similar to those of near isogenic wild-type hybrids. In addition, we report on a 'perfect' PCR marker for selection of the Brrga1-d gene that is based on detecting the nucleotide mutation causing the dwarf phenotype.