Differences in basement membrane-associated microdomains of type I and type II pneumocytes in the rat and rabbit lung.
Differences in basement membrane-associated microdomains of type I and type II pneumocytes in the rat and rabbit lung.
复制标题
大鼠和兔肺中 I 型和 II 型肺细胞基底膜相关微区的差异。
DOI：
--
复制
发表时间：
1984
影响因子：
3.2
通讯作者：
P. Sannes
中科院分区：
文献类型：
--
作者：
P. Sannes
用高铁二胺（HID）技术在电镜水平上观察到，大鼠和兔肺泡I型肺泡上皮细胞的基底膜相关微区与II型肺泡上皮细胞的基底膜相关微区在细胞化学可检测的硫酸酯的特异性分布上存在独特的差异。新生儿和成人肺的醛固定冷冻或振动切片用N，N-二甲基苯二胺-HCl的Meta和帕拉异构体的混合物在氯化铁存在下处理，氯化铁在低pH值（1.0）下先前已被证明对糖胺聚糖和糖蛋白的硫酸酯具有高度特异性。随后用硫代碳酰肼-银蛋白盐增强反应产物，用于电子显微镜检查的植入后序列。观察到以这种方式处理的肺实质样品具有离散的电子致密银颗粒，其与肺基底膜的各种显微解剖成分相关。在肺泡基底膜的区域中，与I型细胞相关的外板中观察到含有丰富的规则布置的、细胞化学可检测的硫酸酯，而致密板和内板中的反应性比较弥散和稀疏。定量地，62%的反应位点发现在基底膜区的I型细胞定位在板Rara外。相比之下，II型细胞的外疏板有不到一半的反应性病灶指示硫酸酯作为I型细胞基底膜的相同区域。HID反应性硫酸酯被发现均匀分布在与II型细胞的基底膜相关的层内。当考虑到肺泡上皮更新的已知模式时，与II型肺细胞相比，I型肺细胞的基底膜相关微区的基底膜相关硫酸酯组合物的这种细胞化学可检测的差异可能是非常显著的。由于已知II型细胞会分裂并保持II型细胞或分化为I型细胞，因此肺泡基底膜及其相关结构的分子组成的区域差异可能是肺泡中细胞分化的细胞特异性过程的关键决定因素。
The basement membrane-associated microdomains of type I pneumocytes in rat and rabbit pulmonary alveoli were found to be uniquely different from those of type II pneumocytes in the specific distribution of cytochemically detectable sulfate esters as demonstrated with the high iron diamine (HID) technique at the electron microscopic level. Aldehyde-fixed frozen or Vibratome sections of neonatal and adult lungs were treated with a mixture of the meta and para isomers of N,N-dimethyl-phenylenediamine-HCl in the presence of ferric chloride, which at low pH (1.0) has been previously shown to be highly specific for sulfate esters of glycosaminoglycans and glycoproteins. Reaction product was subsequently enhanced with a thiocarbohydrazide-silver proteinate, postembedding sequence for electron microscopy. Samples of lung parenchyma treated in this fashion were observed to have discrete, electron-dense silver grains associated with the various microanatomical components of pulmonary basement membranes. In the region of the alveolar basement membrane, the lamina rara externa associated with type I cells was observed to contain an abundance of regularly disposed, cytochemically detectable sulfate esters, while the lamina densa and lamina rara interna were diffusely and sparsely reactive by comparison. Quantitatively, 62% of all reactive sites found in the basement membrane region of type I cells were localized in the lamina rara externa. By contrast, the lamina rara externa of type II cells had less than half as many reactive foci indicative of sulfate esters as the same region of type I cell basement membranes. HID-reactive sulfate esters were found evenly distributed within the laminae associated with the basement membrane of type II cells. This cytochemically detectable difference in the sulfate ester composition of basement membrane-associated sulfate ester composition of basement membrane-associated microdomains of type I compared with that of type II pneumocytes may be highly significant when considering known patterns of epithelial renewal in pulmonary alveoli. Since type II cells are known to divide and either remain type II cells or differentiate into type I cells, regional differences in the molecular composition of the alveolar basement membranes and their associated structures may be key determinants of cell-specific processes of cytodifferentiation in the pulmonary alveolus.
骨骺软骨细胞分泌颗粒中复合碳水化合物的超微结构细胞化学和放射自显影。
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Takagi,M;Parmley,RT;Denys,FR
通讯作者：
Denys,FR
啮齿动物组织中巢蛋白（一种硫酸化基底膜成分）的免疫定位，并与 GP-2（层粘连蛋白）进行比较。
DOI：
--
发表时间：
1981
期刊：
The American journal of pathology
影响因子：
--
作者：
Bender,BL;Jaffe,R;Carlin,B;Chung,AE
通讯作者：
Chung,AE
复杂碳水化合物在成牙本质细胞、前牙本质和牙本质中的超微结构定位。
DOI：
10.1177/29.6.6166667
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Takagi,M;Parmley,RT;Denys,FR
通讯作者：
Denys,FR