A NEW SALMONELLA TESTER STRAIN (TA102) WITH A.T BASE-PAIRS AT THE SITE OF MUTATION DETECTS OXIDATIVE MUTAGENS
A NEW SALMONELLA TESTER STRAIN (TA102) WITH A.T BASE-PAIRS AT THE SITE OF MUTATION DETECTS OXIDATIVE MUTAGENS
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DOI：
10.1073/pnas.79.23.7445
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
AMES, BN
中科院分区：
文献类型：
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作者：
LEVIN, DE;HOLLSTEIN, M;AMES, BN
一种新的试验菌株TA102被描述为鼠伤寒沙门菌/哺乳动物微粒体诱变试验菌株集的新成员。这个菌株包含一个。cntdot。突变位点的T碱基对（通过DNA序列分析确定）与其他沙门氏菌测试菌株相比，检测到突变剂破坏G-C碱基对。该菌株不同于以前的测试菌株，因为突变被引入到多拷贝质粒中，因此。突变基因的30个拷贝可用于反向突变。这种新菌株可以检测各种氧化诱变剂，包括x射线、博来霉素、H2O2和其他氢过氧化物、链霉素和其他醌类以及苯肼；多种醛类，包括甲醛、乙二醛、酮二醛、戊二醛和丙二醛；一些补骨脂素（在近紫外光存在下），丝裂霉素C，新羧化抑素和紫外光。其中一些诱变剂已经被证明可以破坏DNA中的胸腺嘧啶。本文还介绍了几种辅助试验菌株，包括TA96，这是一种移码试验菌株，在5a .cntdot运行时具有突变热点。特异度与TA102相似的T碱基对。讨论了氧化诱变剂的重要性。
A new tester strain, TA102, is described as an addition to the set of strains for the Salmonella typhimurium/mammalian microsome mutagenicity test. This strain contains A .cntdot. T base pairs at the site of the mutation (determined by DNA sequence analysis) in contrast to the other Salmonella tester strains that detect mutagens damaging G-C base pairs. This strain differs from previous tester strains in that the mutation has been introduced into a multicopy plasmid, so that .apprx. 30 copies of the mutant gene are available for back mutation. The new strain detects a variety of oxidative mutagens, including X-rays, bleomycin, H2O2 and other hydroperoxides, streptonigrin and other quinones, and phenylhydrazine; a variety of aldehydes, including formaldehyde, glyoxal, kethoxal, glutaraldehyde and malondialdehyde; a number of psoralens (in the presence of near-UV light), mitomycin C, neocarzinostatin and UV light. Some of these mutagens have been reviously shown to damage thymine in DNA. Several auxiliary tester strains also are described, including TA96, a frameshift tester srain with a hot spot for mutation at a run of 5 A .cntdot. T base pairs with a specificity similar to that of TA102. The importance of oxidative mutagens is discussed.