Membrane-bound immunoglobulins on human leukemic cells. Evidence for humoral immune responses of patients to leukemia-associated antigens.
Membrane-bound immunoglobulins on human leukemic cells. Evidence for humoral immune responses of patients to leukemia-associated antigens.
复制标题
人类白血病细胞上的膜结合免疫球蛋白。
DOI：
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发表时间：
1975
影响因子：
15.9
通讯作者：
D. Miller
中科院分区：
文献类型：
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作者：
R. Metzgar;T. Mohanakumar;D. Miller
用微量细胞毒技术检测了人白血病细胞膜上的免疫球蛋白。一个显着百分比的淋巴细胞从正常供体未能与山羊抗血清的人重链决定簇或轻链。然而，来自一些正常供体的淋巴细胞确实与k-轻链的抗血清反应。来自一些白血病患者的高百分比（50-90）的细胞被轻链抗血清和一种或多种重链决定簇抗血清杀死。胰蛋白酶治疗白血病细胞导致所有免疫球蛋白抗血清的细胞毒活性丧失。胰蛋白酶消化慢性粒细胞白血病（CML）细胞后2小时和急性淋巴细胞白血病（ALL）细胞治疗后8小时，可检测到与k-轻链抗血清的反应性。CML和ALL细胞胰蛋白酶消化后8和48 h，分别检测不到重链决定簇和轻链的抗血清反应性。免疫球蛋白抗血清对重链的细胞毒活性通过与用于通过沉淀确定抗血清的特异性免疫球蛋白的吸收而消除。由白血病细胞制备的洗脱液（pH 3.2）通过细胞毒性与免疫球蛋白抗血清反应，显示含有不同重链类别的免疫球蛋白。此外，部分洗脱液对人白血病细胞具有细胞毒抗体活性。洗脱抗体的特异性类似于先前描述的来自白血病患者的嗜细胞抗体和针对人白血病细胞的非人灵长类动物抗血清的特异性。考虑了洗脱的非补体结合抗体的可能体外检测和体内意义。
Immunoglobulins were detected on the membranes of human leukemic cells by a microcytotoxicity technique. A significant percentage of lymphocytes from normal donors failed to react with goat antisera to human heavy chain determinants or to lambda-light chains. Lymphocytes from some normal donors, however, did react with antisera to k-light chains. A high percentage (50-90) of cells from some leukemia patients were killed by antisera to light chains and by one or more antisera to heavy chain determinants. Trypsin treatment of leukemic cells resulted in a loss of cytotoxic activity with all immunoglobulin antisera. Reactivity with the k-light chain antiserum was detectable 2 h after trypsinization of chronic myeloid leukemic (CML) cells and 8 h after treatment of acute lymphocytic leukemic (ALL) cells. Reactivity with the antisera to heavy chain determinants and lambda-light chains could not be detected 8 and 48 h after trypsinization of CML and ALL cells, respectively. The cytotoxic activity of the immunoglobulin antisera to heavy chains was abolished by absorption with the specific immunoglobulin used to define the antisera by precipitation. Eluates (pH 3.2) prepared from leukemic cells which reacted by cytotoxicity with the immunoglobulin antisera were shown to contain immunoglobulins of different heavy chain classes. In addition, some of the eluates had cytotoxic antibody activity to human leukemia cells. The specificity of the eluted antibodies is similar to the specificity previously described for cytophilic antibodies from leukemic patients and nonhuman primate antisera to human leukemia cells. The possible in vitro detection and in vivo significance of the eluted non-complement-fixing antibodies is considered.