Allium chemistry: Supercritical fluid extraction and LC-APCI-MS of thiosulfinates and related compounds from homogenates of garlic, onion, and ramp. Identification in garlic and ramp and synthesis of 1-propanesulfinothioic acid S-allyl ester
Allium chemistry: Supercritical fluid extraction and LC-APCI-MS of thiosulfinates and related compounds from homogenates of garlic, onion, and ramp. Identification in garlic and ramp and synthesis of 1-propanesulfinothioic acid S-allyl ester
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DOI：
10.1021/jf970314e
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发表时间：
1997-11-01
影响因子：
6.1
通讯作者：
Block, E
中科院分区：
文献类型：
--
作者：
Calvey, EM;Matusik, JE;Block, E
超临界流体（SF）萃取均质大蒜（Allium sativum L.），斜坡（A. tricoccum Ait.），化学电离质谱鉴定。从大蒜和斜坡中提取的主要硫代亚硫酸盐很容易用该技术进行表征。在大蒜匀浆的SP提取物中也发现了少量的阿琼烯，一种有效的抗血栓剂。洋葱汁提取物的谱图揭示了先前研究中报道的常见的硫代亚硫酸盐，zwiebelanes和二亚硫酸盐，以及以前通过广泛的分离步骤和光谱方法在洋葱汁提取物中发现的cepaene。用lc -质谱（MS）验证了洋葱汁中存在微量烯丙基化合物，大蒜和斜坡匀浆中存在微量丙基化合物。这些化合物的存在在以前使用气相色谱-质谱与冷柱上注射和反相或正相LC与紫外检测的分析中不容易明显。
Supercritical fluid (SF) extracts of homogenized garlic (Allium sativum L.), ramp (A. tricoccum Ait.), chemical ionization mass spectrometric identification. The major thiosulfinates from garlic and ramp were readily characterized by this technique. Small quantities of ajoene, a potent antithrombotic agent, were also found in SP extracts of garlic homogenates. The profiles of onion juice extracts revealed the usual thiosulfinates, zwiebelanes, and bissulfine reported in prior studies, as well as cepaenes previously identified in extracts of onion juice through extensive isolation steps and spectroscopic methods. The presence of trace quantities of allyl compounds in onion juice and propyl compounds in garlic and ramp-homogenates has been verified by LC-mass spectrometry (MS). The presence of these compounds was not readily evident in previous analyses using gas chromatography-MS with cold-on-column injection and reversed-phase or normal phase LC with UV detection.