Multiple innervation of Purkinje cells by climbing fibers in the cerebellum of the adult staggerer mutant mouse.
Multiple innervation of Purkinje cells by climbing fibers in the cerebellum of the adult staggerer mutant mouse.
复制标题
通过成年交错突变小鼠小脑中的攀爬纤维对浦肯野细胞进行多重神经支配。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
J. Changeux
中科院分区：
文献类型：
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作者：
Jean Mariani;J. Changeux
成年交错突变小鼠小脑浦肯野细胞(PC)的细胞内记录显示，PC对顶旁(JF)刺激的顺向反应与攀升纤维反应(CFR)非常相似。然而，对于大多数被研究的PC来说，随着刺激强度的增加，这些反应是以一种逐步的方式分级的。兴奋性突触电位(EPSP)具有典型的爬升纤维(CFs)介导的EPSPS形状，但其大小呈离散的阶跃波动，最高达到放电水平。在相同的PC中，自发EPSP的大小以相似的方式波动，每一步的频率都在Cf介导的EPSP的范围内。这些结果强烈地表明，在交错的小鼠中，每台PC上有几个CFs突触，而不是像正常成年人那样只有一个CFs突触。此外，通过其中一些CFs的激活并没有达到相应PC的激发水平。
Intracellular recordings from Purkinje cells (PC) in the cerebellum of adult staggerer mutant mice revealed that the orthodromic response of PCs to juxtafastigial (JF) stimulation closely resembled a climbing fiber response (CFR). However, for most of the PCs studied, these responses were graded in a stepwise manner when the stimulus strength was increased. The underlying excitatory synaptic potentials (EPSPs) had the typical shape of EPSPs mediated through climbing fibers (CFs), but their size fluctuated in discrete steps, the highest one reaching the firing level. In the same PCs, the size of the spontaneous EPSPs fluctuated in a similar fashion and the frequency of each step was in the range of CF-mediated EPSPs. These results strongly suggest that in staggerer mice several CFs synapse with each PC instead of a single CF as in normal adults. Furthermore, the activation through some of these CFs does not reach the firing level of the corresponding PC.