MORPHOLOGICAL AND BIOCHEMICAL-CHANGES IN THE PANCREAS OF THE COPPER-DEFICIENT FEMALE RAT
MORPHOLOGICAL AND BIOCHEMICAL-CHANGES IN THE PANCREAS OF THE COPPER-DEFICIENT FEMALE RAT
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DOI：
10.1093/jn/119.8.1165
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发表时间：
1989-08-01
影响因子：
4.2
通讯作者：
MAJUMDAR, APN
中科院分区：
文献类型：
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作者：
DUBICK, MA;YU, GSM;MAJUMDAR, APN
早期的组织学研究表明，铜缺乏导致胰腺腺泡组织的选择性和进行性萎缩。本研究探讨了营养性铜缺乏时胰腺外分泌的生化和形态学变化。给成年雌性大鼠组喂食铜不足（< 0.5 μ g/g）或铜充足（6.2 μ g/g）的纯化饮食6周。铜缺乏导致腺泡细胞的超微结构发生明显变化，包括酶原颗粒含量的显著变化、自噬空泡和腺泡腔的扩张。胰腺重量和总DNA，RNA和蛋白质含量在两组大鼠中是相似的，而胰腺淀粉酶，胰蛋白酶和胰凝乳蛋白酶的活性显着较低的铜缺乏组。此外，促分泌素诱导的释放这些酶从分散的腺泡分离铜缺乏大鼠显着减少相比，正常对照组的酶分泌。胰腺铜锌和锰超氧化物歧化酶活性也被发现在铜缺乏大鼠显着低于正常对照组。我们的结论是，营养性铜缺乏症在成年雌性大鼠胰腺分泌素的反应性降低，并可能增加胰腺氧化损伤的易感性。
Earlier histological studies have demonstrated that copper deficiency results in a selective and progressive atrophy of pancreatic acinar tissue. The present study examines both biochemical and morphological changes of the exocrine pancreas in nutritional copper deficiency. Groups of mature female rats were fed a purified diet either deficient (< 0.5 .mu.g/g) or sufficient (6.2 .mu.g/g) in copper for 6 wk. Copper deficiency resulted in distinct ultrastructural changes in acinar cells, including marked variability in zymogen granule content, autophagic vacuoles and dilation of acinar lumen. Pancreatic weight and total DNA, RNA and protein content of the pancreas were similar in both groups of rats, whereas pancreatic amylase, trypsin and chymotrypsin activity was significantly lower in the copper-deficient group. In addition, secretagogue-induced release of these enzymes from dispersed acini isolated from copper-deficient rats was significantly reduced in comparison to enzyme secretion from normal controls. Pancreatic Cu-Zn and Mn superoxide dismutase activity was also found to be significantly lower in the copper-deficient rats than in normal controls. We conclude that nutritional copper deficiency in adult female rats reduces the responsiveness of the pancreas to secretagogues and may increase the susceptibility of the pancreas to oxidative damage.