Pseudomonas aeruginosa anaerobic respiration in biofilms:: Relationships to cystic fibrosis pathogenesis
Pseudomonas aeruginosa anaerobic respiration in biofilms:: Relationships to cystic fibrosis pathogenesis
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DOI：
10.1016/s1534-5807(02)00295-2
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发表时间：
2002-10-01
影响因子：
11.8
通讯作者：
Hassett, DJ
中科院分区：
文献类型：
--
作者：
Yoon, SS;Hennigan, RF;Hassett, DJ
最近的资料表明，囊性纤维化（CF）气道粘液是厌氧的。这表明CF中的铜绿假单胞菌感染反映了生物膜的形成和在厌氧环境中的持久性。P. aeruginosa形成了坚固的厌氧生物膜，其生存能力需要rhl群体感应和一氧化氮（NO）还原酶来调节或防止有毒NO的积累，NO是厌氧呼吸的副产物。蛋白质组学分析发现了一种外膜蛋白OprF，在厌氧条件下与有氧条件下上调了40倍。此外，CF黏液中存在OprF， CIF患者可提高OprF的抗血清水平。oprF突变体形成了较差的厌氧生物膜，部分原因是厌氧呼吸缺陷。因此，未来对CF发病机制和治疗的研究应包括更好地了解P. aeruginosa的厌氧代谢和生物膜发育。
Recent data indicate that cystic fibrosis (CF) airway mucus is anaerobic. This suggests that Pseudomonas aeruginosa infection in CF reflects biofilm formation and persistence in an anaerobic environment. P. aeruginosa formed robust anaerobic biofilms, the viability of which requires rhl quorum sensing and nitric oxide (NO) reductase to modulate or prevent accumulation of toxic NO, a byproduct of anaerobic respiration. Proteomic analyses identified an outer membrane protein, OprF, that was upregulated similar to40-fold under anaerobic versus aerobic conditions. Further, OprF exists in CF mucus, and CIF patients raise antisera to OprF. An oprF mutant formed poor anaerobic biofilms, due, in part, to defects in anaerobic respiration. Thus, future investigations of CF pathogenesis and therapy should include a better understanding of anaerobic metabolism and biofilm development by P. aeruginosa.